{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T23:28:24Z","timestamp":1782775704769,"version":"3.54.5"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T00:00:00Z","timestamp":1546992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["16POST30990070"],"award-info":[{"award-number":["16POST30990070"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL120140"],"award-info":[{"award-number":["R01HL120140"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HD096335"],"award-info":[{"award-number":["R01HD096335"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:p>In mammals, preimplantation development primarily occurs in the oviduct (or fallopian tube) where fertilized oocytes migrate through, develop and divide as they prepare for implantation in the uterus. Studies of preimplantation development currently rely on ex vivo experiments with the embryos cultured outside of the oviduct, neglecting the native environment for embryonic growth. This prevents the understanding of the natural process of preimplantation development and the roles of the oviduct in early embryonic health. Here, we report an in vivo optical imaging approach enabling high\u2010resolution visualizations of developing embryos in the mouse oviduct. By combining optical coherence microscopy (OCM) and a dorsal imaging window, the subcellular structures and morphologies of unfertilized oocytes, zygotes and preimplantation embryos can be well resolved in vivo, allowing for the staging of development. We present the results together with bright\u2010field microscopy images to show the comparable imaging quality. As the mouse is a well\u2010established model with a variety of genetic engineering strategies available, the in vivo imaging approach opens great opportunities to investigate how the oviduct and early embryos interact to prepare for successful implantation. This knowledge could have beneficial impact on understanding infertility and improving in vitro fertilization. OCM through a dorsal imaging window enables high\u2010resolution imaging and staging of mouse preimplantation embryos in vivo in the oviduct.<\/jats:p><jats:p><jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio201800364-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.201800364","type":"journal-article","created":{"date-parts":[[2018,12,22]],"date-time":"2018-12-22T04:05:39Z","timestamp":1545451539000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Staging mouse preimplantation development in vivo using optical coherence microscopy"],"prefix":"10.1002","volume":"12","author":[{"given":"Emma L.","family":"Moore","sequence":"first","affiliation":[{"name":"Department of Molecular Physiology and Biophysics Baylor College of Medicine  Houston Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics Baylor College of Medicine  Houston Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1571-2997","authenticated-orcid":false,"given":"Irina V.","family":"Larina","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics Baylor College of Medicine  Houston Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.060426"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1808"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.2010.01172.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2014.08.028"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-15-0180"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128314"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.fluid.37.061903.175725"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-16-0302"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00190-7"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1364\/AO.51.003650"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.17.7.070503"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.18.1.010503"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.20.9.096004"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-04220-8"},{"key":"e_1_2_7_16_1","first-page":"865","volume-title":"Optical Coherence Microscopy","author":"Aguirre A. D.","year":"2015"},{"key":"e_1_2_7_17_1","first-page":"791","volume-title":"Time Domain Full Field Optical Coherence Tomography Microscopy","author":"Harms F.","year":"2015"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.6.002713"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13216"},{"key":"e_1_2_7_20_1","volume":"145","author":"Wang S.","year":"2018","journal-title":"Development"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201700316"},{"key":"e_1_2_7_22_1","unstructured":"C. Caligioni. Curr. Protoc. Neurosci. 2009. Editorial board Jacqueline N. Crawley \u2026 [et al.]. APPENDIX Appendix\u20104I."},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-210-6_9"},{"key":"e_1_2_7_24_1","volume-title":"Manipulating the Mouse Embryo: A Laboratory Manual","author":"Behringer R.","year":"2014"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0512-6_12"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.116.140400"},{"key":"e_1_2_7_27_1","first-page":"539","volume":"23","author":"Modli\u0144ski J. A.","year":"1970","journal-title":"J. Embryol. Exp. 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