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This study evaluates Raman spectroscopy (RS) to identify spectral characteristics of infection and differentiate GBS from <jats:italic>Escherichia coli<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic> during ex vivo infection of human fetal membrane tissues. Unique spectral features were identified from colonies grown on agar and infected fetal membrane tissues. Multinomial logistic regression analysis accurately identified GBS infected tissues with 100.0% sensitivity and 88.9% specificity. Together, these findings support further investigation into the use of RS as an emerging microbiologic diagnostic tool and intrapartum screening test for GBS carriage.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio201800449-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.201800449","type":"journal-article","created":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T17:13:29Z","timestamp":1559668409000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2691-7058","authenticated-orcid":false,"given":"Oscar D.","family":"Ayala","sequence":"first","affiliation":[{"name":"Biophotonics Center Vanderbilt University  Nashville Tennessee"},{"name":"Department of Biomedical Engineering Vanderbilt University  Nashville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan S.","family":"Doster","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases Vanderbilt University Medical Center  Nashville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannon D.","family":"Manning","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics Michigan State University  East Lansing Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine M.","family":"O'Brien","sequence":"additional","affiliation":[{"name":"Biophotonics Center Vanderbilt University  Nashville Tennessee"},{"name":"Department of Biomedical Engineering Vanderbilt University  Nashville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Aronoff","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases Vanderbilt University Medical Center  Nashville Tennessee"},{"name":"Department of Pathology, Microbiology and Immunology Vanderbilt University Medical Center  Nashville Tennessee"},{"name":"Department of Obstetrics and Gynecology Vanderbilt University Medical Center  Nashville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer A.","family":"Gaddy","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases Vanderbilt University Medical Center  Nashville Tennessee"},{"name":"Department of Pathology, Microbiology and Immunology Vanderbilt University Medical Center  Nashville Tennessee"},{"name":"Department of Veterans Affairs Tennessee Valley Healthcare Systems  Nashville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita","family":"Mahadevan\u2010Jansen","sequence":"additional","affiliation":[{"name":"Biophotonics Center Vanderbilt University  Nashville Tennessee"},{"name":"Department of Biomedical Engineering Vanderbilt University  Nashville Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix585"},{"issue":"10","key":"e_1_2_7_3_1","first-page":"1","volume":"59","author":"Verani J.","year":"2010","journal-title":"MMWR Recomm. 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