{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T06:39:09Z","timestamp":1756190349030,"version":"3.41.2"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T00:00:00Z","timestamp":1574208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Irradiation of femtosecond (fs) pulse lasers in the visible and near\u2010infrared ranges have been proposed as a promising approach for inactivating viruses. However, in order to achieve significant virus inactivation, past works have required relatively long irradiation times (1 hour or longer), even for small volumes. Given its advantages compared with other techniques, there is an urgent need to shorten the time required to inactivate viruses using fs laser technology. In this study, we investigate the inactivation of purified M13 bacteriophage in phosphate\u2010buffered saline with large active volume (1 cm<jats:sup>3<\/jats:sup>), and short exposure time (several minutes), using lasers with 20\u2009mJ\/pulse energy at various wavelengths (800, 400\u2009nm or both 800 and 400\u2009nm combined). For an exposure time of 15 and 2 minute, the use of a 400\u2009nm wavelength laser results in a high load reduction of 5.8\u2009\u00b1\u20090.3 and 2.9\u2009\u00b1\u20090.15, respectively, on the log<jats:sub>10<\/jats:sub> scale of viability. We show that virus inactivation using the 400\u2009nm laser is much more efficient compared with that using an 800\u2009nm laser, or the simultaneous irradiation of 400 and 800\u2009nm lasers. Higher pathogen inactivation is observed for lasers with shorter pulse duration, whereas at longer pulse durations, the inactivation is reduced. For millijoule\u2010energy fs laser irradiation, the M13 bacteriophage inactivation, via the reduction of the functionality of M13 bacteriophages, is accompanied with relatively small amounts of genetic damage.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio201900001-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.201900001","type":"journal-article","created":{"date-parts":[[2019,10,26]],"date-time":"2019-10-26T02:38:00Z","timestamp":1572057480000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Accelerated inactivation of M13 bacteriophage using millijoule femtosecond lasers"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0689-8547","authenticated-orcid":false,"given":"Aziz","family":"Berchtikou","sequence":"first","affiliation":[{"name":"INRS\u2013Centre \u00c9nergie Mat\u00e9riaux T\u00e9l\u00e9communications  Qu\u00e9bec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea A.","family":"Greschner","sequence":"additional","affiliation":[{"name":"INRS\u2013Centre \u00c9nergie Mat\u00e9riaux T\u00e9l\u00e9communications  Qu\u00e9bec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Tijssen","sequence":"additional","affiliation":[{"name":"INRS\u2013Centre Institut Armand\u2010Frappier  Qu\u00e9bec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc A.","family":"Gauthier","sequence":"additional","affiliation":[{"name":"INRS\u2013Centre \u00c9nergie Mat\u00e9riaux T\u00e9l\u00e9communications  Qu\u00e9bec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuneyuki","family":"Ozaki","sequence":"additional","affiliation":[{"name":"INRS\u2013Centre \u00c9nergie Mat\u00e9riaux T\u00e9l\u00e9communications  Qu\u00e9bec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,11,20]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.moh.0000137915.88478.23"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2010.01374.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-2824.2011.01471.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2008.01.001"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.3275477"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1088\/0953-8984\/19\/47\/472201"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-19-62"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-11-20"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1117\/12.791305"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1109\/JSTQE.2015.2498920"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1260\/2040-2295.1.2.185"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.3600771"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1088\/0953-8984\/20\/25\/252205"},{"key":"e_1_2_5_15_1","first-page":"228","volume":"42","author":"Ling T. C.","year":"2004","journal-title":"J. Microbiol"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4018(85)90151-8"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1364\/AO.43.000883"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1148286"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-63.2.190"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-73.9.1633"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.2424"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-3-79"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.449708"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1088\/0953-8984\/21\/50\/505102"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.80.041909"},{"key":"e_1_2_5_26_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1088\/978-1-6817-4353-0","volume-title":"Selective Photonic Disinfection: A Ray of Hope in the War against Pathogens","author":"Tsen S.\u2010W. D.","year":"2016"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.6b00545"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr00025a006"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1088\/2399-1984\/aadf9d"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-12377-5"},{"issue":"4","key":"e_1_2_5_31_1","first-page":"2548","volume":"2","author":"Nazari M.","year":"2019","journal-title":"Appl. Nanomater."},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2005.04.067"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2008.01233.x"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1109\/27.842870"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2003.tb00644.x"},{"key":"e_1_2_5_36_1","first-page":"96","volume-title":"Chemical Reactions and Their Control on the Femtosecond Time Scale. 20th Advances in Chemical Physics, Volume 101","author":"Gaspard P.","year":"1997"},{"key":"e_1_2_5_37_1","first-page":"256","volume-title":"Molecular Orbital Calculations for Biological Systems","author":"Sapse A.\u2010M.","year":"1998"},{"key":"e_1_2_5_38_1","first-page":"371","volume-title":"Biological and Medical Aspects of Electromagnetic Fields","author":"Barnes F. S.","year":"2006"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtbi.2006.01.028"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1088\/0022-3727\/47\/31\/315402"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1089\/104454702320901116"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.5978\/islsm.20.17"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2006.24.680"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13346-015-0260-0"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-018-1019-2"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2009.00897.x"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjbio.201900001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.201900001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbio.201900001","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.201900001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T05:54:37Z","timestamp":1694152477000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.201900001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,20]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1002\/jbio.201900001"],"URL":"https:\/\/doi.org\/10.1002\/jbio.201900001","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"type":"print","value":"1864-063X"},{"type":"electronic","value":"1864-0648"}],"subject":[],"published":{"date-parts":[[2019,11,20]]},"assertion":[{"value":"2019-01-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e201900001"}}