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However, keratinocyte nuclei have been reported to be either low scattering or high scattering, so that these inconsistent reports might have led to misinterpretations of optical images, and more importantly, hindered the establishment of optical diagnostic criteria. We disclose that they are generally low scattering in the core using Micro\u2010optical coherence tomography (\u03bcOCT) of 1.28\u2010\u03bcm axial resolution in vivo; those previously reported \u201chigh scattering\u201d or \u201cbright\u201d signals from nuclei are likely from the nucleocytoplasmic boundary, and the low\u2010scattering nuclear cores were missed possibly due to insufficient axial resolutions (~4\u03bcm). It is further demonstrated that the high scattering signals may be associated with flattening of nuclei and cytoplasmic glycogen accumulation, which are valuable cytologic hallmarks of cell maturation.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio201900073-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.201900073","type":"journal-article","created":{"date-parts":[[2019,5,17]],"date-time":"2019-05-17T16:20:31Z","timestamp":1558110031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Contrast of nuclei in stratified squamous epithelium in optical coherence tomography images at 800 nm"],"prefix":"10.1002","volume":"12","author":[{"given":"Si","family":"Chen","sequence":"first","affiliation":[{"name":"School of Electrical and Electronic Engineering Nanyang Technological University  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyu","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Electrical and Electronic Engineering Nanyang Technological University  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nanshuo","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Electrical and Electronic Engineering Nanyang Technological University  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianshan","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianghong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Electrical and Electronic Engineering Nanyang Technological University  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Ge","sequence":"additional","affiliation":[{"name":"School of Electrical and Electronic Engineering Nanyang Technological University  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"En","family":"Bo","sequence":"additional","affiliation":[{"name":"School of Electrical and Electronic Engineering Nanyang Technological University  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojun","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Automation Northwestern Polytechnical University  Xi'an Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honggang","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenjie","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Chemical and Biomedical Engineering Nanyang Technological University  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6691-9253","authenticated-orcid":false,"given":"Linbo","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Electrical and Electronic Engineering Nanyang Technological University  Singapore"},{"name":"School of Chemical and Biomedical Engineering Nanyang Technological University  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12606215"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00690.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2000.104844"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2005.07.049"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gie.2006.10.054"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.20911"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gie.2006.04.027"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt892"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1450"},{"key":"e_1_2_8_11_1","volume":"14","author":"Yuan Z.","year":"2009","journal-title":"J. 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