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However, the self\u2010assembly mechanism of lipid raft cannot be revealed even its physical properties and components were determined in specific physiological processes. In this study, two\u2010photon generalised polarisation imaging and fluorescence correlation spectroscopy were used to study the fusion of lipid rafts through the membrane phase and the lateral diffusion of lipids in living breast cancer cells. A self\u2010assembly model of lipid rafts associated with lipid diffusion and membrane phase was proposed to demonstrate the lipid sorting ability of lipid rafts in the plasma membrane. The results showed that the increased proportion of slow subdiffusion of G<jats:sub>M1<\/jats:sub>\u2010binding cholera toxin B\u2010subunit (CT\u2010B) was accompanied with an increased liquid\u2010ordered domain during the \u03b2\u2010estradiol\u2010induced fusion of lipid rafts. And slow subdiffusion of CT\u2010B was vanished with the depletion of lipid rafts. Whereas the dialkylindocarbocyanine (DiIC<jats:sub>18<\/jats:sub>) diffusion was not specifically regulated by lipid rafts. This study will open up a new insight for uncovering the self\u2010assembly of lipid rafts in specific pathophysiological processes.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio201900214-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.201900214","type":"journal-article","created":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T14:48:17Z","timestamp":1572619697000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Self\u2010assembly of lipid rafts revealed by fluorescence correlation spectroscopy in living breast cancer cells"],"prefix":"10.1002","volume":"13","author":[{"given":"Shiqing","family":"Dong","sequence":"first","affiliation":[{"name":"Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Provincial Key Laboratory for Photonics Technology Fujian Normal University  Fuzhou 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