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Age\u2010related renal insufficiency has important implications with regard to body homeostasis, drug toxicity and renal transplantation. In our study, two\u2010photon microscopy was used to image kidney morphological and functional characteristics in an age\u2010related rat model in vivo. The changes in morphology are analyzed based on autofluorescence and Hoechst 33342 labeling in rats with different ages. Structural parameters including renal tubular diameter, cell nuclei density, size and shape are studied and compared with Hematoxylin and Eosin histological analysis. Functional characteristics, such as blood flow, and glomerular filtration rate are studied with high\u2010molecular weight (MW) 500\u2010kDa dextran\u2010fluorescein and low\u2010MW 10\u2010kDa dextran\u2010rhodamine. Results indicate that morphology changes significantly and functional characteristics deteriorate with age. These parameters are potential indicators for evaluating age\u2010related renal morphology and function changes. Combined analyses of these parameters could provide a quantitative, novel method for monitoring kidney diseases and\/or therapeutic effects of kidney drugs.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio201900246-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.201900246","type":"journal-article","created":{"date-parts":[[2019,11,5]],"date-time":"2019-11-05T16:32:19Z","timestamp":1572971539000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Morphological and functional characteristics of aging kidneys based on two\u2010photon microscopy in vivo"],"prefix":"10.1002","volume":"13","author":[{"given":"Shulian","family":"Wu","sequence":"first","affiliation":[{"name":"Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education  Fuzhou China"},{"name":"Fischell Department of Bioengineering University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hengchang","family":"Guo","sequence":"additional","affiliation":[{"name":"Fischell Department of Bioengineering University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Horng","sequence":"additional","affiliation":[{"name":"Fischell Department of Bioengineering University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Fischell Department of Bioengineering University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parnaz","family":"Daneshpajouhnejad","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins Medical Institutions  Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Avi","family":"Rosenberg","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins Medical Institutions  Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Albanese","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular &amp; 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