{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:31:12Z","timestamp":1790832672392,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,9]],"date-time":"2019-10-09T00:00:00Z","timestamp":1570579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AR072946"],"award-info":[{"award-number":["R21AR072946"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pathological crystal identification is routinely practiced in rheumatology for diagnosing arthritis disease such as gout, and relies on polarized light microscopy as the gold standard method used by medical professionals. Here, we present a single\u2010shot computational polarized light microscopy method that reconstructs the transmittance, retardance and slow\u2010axis orientation of a birefringent sample using a single image captured with a pixelated\u2010polarizer camera. This method is fast, simple\u2010to\u2010operate and compatible with all the existing standard microscopes without extensive or costly modifications. We demonstrated the success of our method by imaging three different types of crystals found in synovial fluid and reconstructed the birefringence information of these samples using a single image, without being affected by the orientation of individual crystals within the sample field\u2010of\u2010view. We believe this technique will provide improved sensitivity, specificity and speed, all at low cost, for clinical diagnosis of crystals found in synovial fluid and other bodily fluids.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio201960036-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.201960036","type":"journal-article","created":{"date-parts":[[2019,9,4]],"date-time":"2019-09-04T16:27:55Z","timestamp":1567614475000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Pathological crystal imaging with single\u2010shot computational polarized light microscopy"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4744-996X","authenticated-orcid":false,"given":"Bijie","family":"Bai","sequence":"first","affiliation":[{"name":"Electrical and Computer Engineering Department University of California, Los Angeles  Los Angeles California"},{"name":"Bioengineering Department University of California, Los Angeles  Los Angeles California"},{"name":"California NanoSystems Institute University of California, Los Angeles  Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0110-5624","authenticated-orcid":false,"given":"Hongda","family":"Wang","sequence":"additional","affiliation":[{"name":"Electrical and Computer Engineering Department University of California, Los Angeles  Los Angeles California"},{"name":"Bioengineering Department University of California, Los Angeles  Los Angeles California"},{"name":"California NanoSystems Institute University of California, Los Angeles  Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tairan","family":"Liu","sequence":"additional","affiliation":[{"name":"Electrical and Computer Engineering Department University of California, Los Angeles  Los Angeles California"},{"name":"Bioengineering Department University of California, Los Angeles  Los Angeles California"},{"name":"California NanoSystems Institute University of California, Los Angeles  Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yair","family":"Rivenson","sequence":"additional","affiliation":[{"name":"Electrical and Computer Engineering Department University of California, Los Angeles  Los Angeles California"},{"name":"Bioengineering Department University of California, Los Angeles  Los Angeles California"},{"name":"California NanoSystems Institute University of California, Los Angeles  Los Angeles California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"FitzGerald","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Internal Medicine, David Geffen School of Medicine University of California, Los Angeles  Los Angeles 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