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This approach directly demonstrates increased tissue fluid content in ACD lesional skin with a spectral signature that matches the spectral signature of intradermally injected normal saline. The best contrast between the affected and unaffected skin is achieved through a selection of specific wavelengths at 1070, 1340 and 1605\u2009nm and combining them in a pseudo\u2010red\u2010green\u2010blue color space. An image derived from these wavelengths normalized to unaffected skin defines a \u201ctissue fluid index\u201d that may aid in the quantitative diagnosis and monitoring of ACD. Further clinical testing of this promising approach towards disease detection and monitoring with tissue fluid content quantification is warranted.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202000040-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202000040","type":"journal-article","created":{"date-parts":[[2020,5,17]],"date-time":"2020-05-17T05:29:42Z","timestamp":1589693382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Hyperspectral imaging and characterization of allergic contact dermatitis in the short\u2010wave infrared"],"prefix":"10.1002","volume":"13","author":[{"given":"Tommy","family":"Du","sequence":"first","affiliation":[{"name":"Department of Radiology Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deependra K.","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Radiology Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonid","family":"Shmuylovich","sequence":"additional","affiliation":[{"name":"Department of Radiology Washington University School of Medicine  St. Louis Missouri USA"},{"name":"Division of Dermatology Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Radiology Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Hurbon","sequence":"additional","affiliation":[{"name":"Department of Radiology Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven T.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Radiology Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2670-2487","authenticated-orcid":false,"given":"Mikhail Y.","family":"Berezin","sequence":"additional","affiliation":[{"name":"Department of Radiology Washington University School of Medicine  St. Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2019.08.012"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.det.2011.08.004"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2015.05.001"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/srt.12199"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.19.1.010901"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.18.2.026010"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-54139-5"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8010036"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.9.006283"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.21.12.126006"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0846.2009.00412.x"},{"key":"e_1_2_10_13_1","unstructured":"https:\/\/www.deviceranks.com\/en\/camera-sensor\/59\/sony-imx260-exmor-rs."},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.27.000969"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/s8095576"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(99)00125-9"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep15919"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1364\/JOSAA.21.001231"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cem.938"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.18.10.101318"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0846.2012.00622.x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.20.3.030901"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700329"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.210369597"},{"key":"e_1_2_10_25_1","first-page":"265","volume":"2","author":"Ibraheem N. 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