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Pathophysiology of its diseases is still an interesting issue to be studied since <jats:italic>T gondii<\/jats:italic> can infect nearly all nucleated cells. Imaging techniques are crucial for studying its pathophysiology. In <jats:italic>T gondii\u2010<\/jats:italic>infected cells structural and biochemical alterations occurred. To study that modification, we use digital holotomography to investigate the structure and biochemical alteration of single tachyzoite and its infected cells in a label\u2010free and quantitative manner. Quantification analysis was done by measuring the refractive index distribution, which provides information about the concentration and dry mass of individual cells. This study showed that holotomography could be effectively used to identify the structural and biochemical alteration in tremendously different cells in supporting pathophysiological research in particular for <jats:italic>T gondii\u2010<\/jats:italic>caused diseases.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202000055-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202000055","type":"journal-article","created":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T09:48:49Z","timestamp":1590140929000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>3D<\/scp> morphological and biophysical changes in a single tachyzoite and its infected cells using three\u2010dimensional quantitative phase imaging"],"prefix":"10.1002","volume":"13","author":[{"given":"Egy Rahman","family":"Firdaus","sequence":"first","affiliation":[{"name":"Department of Medical Environmental Biology and Tropical Medicine, School of Medicine Kangwon National University  Chuncheon Gangwon\u2010do Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Hoon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Medical Environmental Biology and Tropical Medicine, School of Medicine Kangwon National University  Chuncheon Gangwon\u2010do Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong\u2010Kyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Medical Environmental Biology and Tropical Medicine, School of Medicine Kangwon National University  Chuncheon Gangwon\u2010do Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YongKeun","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Physics Korea Advanced Institute of Science and Technology (KAIST)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang\u2010Ho","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Medical Science &amp; 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