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Thus, imaging method suitable for expeditious acquisition of the entire mouse penis with subcellular resolution is described that relies on both aqueous\u2010 (clear, unobstructed brain imaging cocktails and computational analysis) and solvent\u2010based (fluorescence\u2010preserving capability imaging of solvent\u2010cleared organs) tissue optical clearing (TOC). The combined TOC approach allows to image mouse penis innervation and vasculature with unprecedented detail and, for the first time, reveals the three\u2010dimensional structure of murine penis fibrocartilage.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202000072-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202000072","type":"journal-article","created":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T17:48:34Z","timestamp":1588268914000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Tissue clearing\u2010based method for unobstructed three\u2010dimensional imaging of mouse penis with subcellular resolution"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3191-510X","authenticated-orcid":false,"given":"Pawe\u0142","family":"Matryba","sequence":"first","affiliation":[{"name":"Department of Immunology Medical University of Warsaw  Warsaw Poland"},{"name":"The Doctoral School of the Medical University of Warsaw Medical University of Warsaw  Warsaw Poland"},{"name":"Laboratory of Neurobiology, BRAINCITY Nencki Institute of Experimental Biology of Polish Academy of Sciences  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artur","family":"Wolny","sequence":"additional","affiliation":[{"name":"Laboratory of Imaging Tissue Structure and Function Nencki Institute of Experimental Biology of Polish Academy of Sciences  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Paw\u0142owska","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology, BRAINCITY Nencki Institute of Experimental Biology of Polish Academy of Sciences  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sosnowska","sequence":"additional","affiliation":[{"name":"Department of Immunology Medical University of Warsaw  Warsaw Poland"},{"name":"Postgraduate School of Molecular Medicine Medical University of Warsaw  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzanna","family":"Rydzy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Immunology Medical University of Warsaw  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Jasi\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Immunology Medical University of Warsaw  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzena","family":"Stefaniuk","sequence":"additional","affiliation":[{"name":"Laboratory of Neurobiology, BRAINCITY Nencki Institute of Experimental Biology of Polish Academy of Sciences  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"Go\u0142\u0105b","sequence":"additional","affiliation":[{"name":"Department of Immunology Medical University of Warsaw  Warsaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10508-015-0484-6"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.13395"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2008.00955.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.111.091504"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000431010"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12387"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/lpor.201800292"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.03.042"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aau8355"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0501-0"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.119"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.10.010"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3964"},{"key":"e_1_2_10_16_1","volume":"12","author":"Li Y.","year":"2018","journal-title":"Front. 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