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To improve the clinical interpretation of OCTA, the conditions affecting visualization of microvessels must be defined. Here we inject a scattering plasma tracer (Intralipid) during OCTA imaging of the anesthetized rat eye. In the retina, we find that interlaminar (vertical) vessels that connect laminae have one\u2010fourth to one\u2010third the OCTA red blood cell to tracer (RBC\u2010to\u2010tracer) signal ratio of intralaminar (horizontal) vessels. This finding suggests that the OCTA signal from microvessels depends on angular orientation, making vertically\u2010oriented vessels more difficult to visualize using intrinsic contrast alone. Clinicians should be aware of this potential artifact when interpreting OCTA.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202000090-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202000090","type":"journal-article","created":{"date-parts":[[2020,5,29]],"date-time":"2020-05-29T08:21:45Z","timestamp":1590740505000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Visibility of microvessels in Optical Coherence Tomography angiography depends on angular orientation"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7905-7856","authenticated-orcid":false,"given":"Jun","family":"Zhu","sequence":"first","affiliation":[{"name":"Biomedical Engineering Department University of California Davis  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel T.","family":"Bernucci","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Department University of California Davis  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conrad W.","family":"Merkle","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Department University of California Davis  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek J.","family":"Srinivasan","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Department University of California Davis  Davis California USA"},{"name":"Department of Ophthalmology and Vision Science University of California Davis School of Medicine  Sacramento California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1307315110"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1500185112"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.2017.181"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2017.07.002"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.2.000781"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaophthalmol.2014.3616"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2018.02.006"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.3653235"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2012.06.054"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.9.001020"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2015.10.017"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2014.08.051"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.35.000043"},{"key":"e_1_2_8_15_1","first-page":"130","volume-title":"International Conference on Medical Image Computing and Computer\u2010Assisted Intervention","author":"Frangi A. 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