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However, cross\u2010sectioning requires tissue destruction and is not practical in situ. In this work, Raman spectroscopy\u2010based multivariate curve resolution (MCR) was employed in porcine cartilage samples (n = 12) to measure collagen, glycosaminoglycan, and water distributions through the surface for the first time; these were compared against cross\u2010section standards. Through the surface Raman measurements proved reliable in predicting composition distribution up to a depth of approximately 0.5\u2009mm. A fructose\u2010based optical clearing agent (OCA) was also used in an attempt to further improve depth of resolution of this measurement method. However, it did not; mainly due to a high\u2010spectral overlap with the Raman spectra of main cartilage sub\u2010components. This measurement technique potentially could be used in situ, to better understand the etiology of joint diseases such as osteoarthritis (OA).<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202000289-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202000289","type":"journal-article","created":{"date-parts":[[2020,10,12]],"date-time":"2020-10-12T07:12:01Z","timestamp":1602486721000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Measurement of cartilage sub\u2010component distributions through the surface by Raman spectroscopy\u2010based multivariate analysis"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1558-0971","authenticated-orcid":false,"given":"Daniel","family":"Mason","sequence":"first","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Carleton University  Ottawa Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangeeta","family":"Murugkar","sequence":"additional","affiliation":[{"name":"Department of Physics Carleton University  Ottawa Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0187-6162","authenticated-orcid":false,"given":"Andrew D.","family":"Speirs","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering Carleton University  Ottawa Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1941738109350438"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-018-0042-7"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.6b00222"},{"key":"e_1_2_9_5_1","first-page":"3","volume":"83","author":"Pavlou E.","year":"2018","journal-title":"Ann. 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