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The self\u2010developed compact ATR microfluidic system was employed to detect two groups of base\u2010repeated DNA molecules combined with a terahertz time\u2010domain spectrometer in a marker\u2010free manner. The first group featured different repetition patterns of oligonucleotide fragments, and the second group included the HD gene. For the oligonucleotides of different repetition patterns, there were significant differences among the three oligonucleotides with three repeats of the double bases, which could be unambiguously classified and identified; For the HD gene, it was found that the magnitude of the terahertz absorption coefficients of the four oligonucleotide solutions was, in ascending order, CAG\u20104\u2009&lt;\u2009CAG\u201016\u2009&lt;\u2009CAG\u201032\u2009&lt;\u2009CAG\u201040 (the numbers are the repeat times of the CAG base segment, with 40 repeats belonging to the HD gene), when the concentration of oligonucleotide was 1 mg\/mL. Principal component analysis result indicated that the spectral differences of the four oligonucleotide solutions with different CAG repeat times were statistically significant and clearly distinguishable. These results demonstrate the potential of terahertz spectroscopy as a noninvasive, unmarked, fast and low\u2010cost assay for gene diagnosis and clinical disease detection.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202000315-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202000315","type":"journal-article","created":{"date-parts":[[2020,9,27]],"date-time":"2020-09-27T16:34:49Z","timestamp":1601224489000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Detection of gene mutation responsible for Huntington's disease by terahertz attenuated total reflection microfluidic spectroscopy"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8170-5824","authenticated-orcid":false,"given":"Mingjie","family":"Tang","sequence":"first","affiliation":[{"name":"Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences  Chongqing China"},{"name":"University of Chinese Academy of Sciences  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingkun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangping","family":"Xia","sequence":"additional","affiliation":[{"name":"Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences  Chongqing China"},{"name":"Key Laboratory of Micro Nano Optoelectronic Devices and Intelligent Perception Systems Yangtze Normal University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongshan","family":"Wei","sequence":"additional","affiliation":[{"name":"Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences  Chongqing China"},{"name":"School of Electronic Engineering Dongguan University of Technology  Dongguan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongbo","family":"Yang","sequence":"additional","affiliation":[{"name":"Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shihan","family":"Yan","sequence":"additional","affiliation":[{"name":"Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunlei","family":"Du","sequence":"additional","affiliation":[{"name":"Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Liang","family":"Cui","sequence":"additional","affiliation":[{"name":"Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-016-8327-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2016.07.012"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30428"},{"key":"e_1_2_7_5_1","first-page":"10","volume":"7568","author":"Rahman A.","year":"2010","journal-title":"Proc. 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