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This process however damages islet basement membranes, which can lead to islet dysfunction or death. Posttransplantation, islets are further stressed by a hypoxic environment and immune reactions that cause poor engraftment and graft failure. The current standards to assess islet quality before transplantation are destructive procedures, performed on a small islet population that does not reflect the heterogeneity of large isolated islet batches. In this study, we incorporated fluorescence lifetime imaging microscopy (FLIM) into a pancreas\u2010on\u2010chip system to establish a protocol to noninvasively assess the viability and functionality of pancreatic \u03b2\u2010cells in a three\u2010dimensional in vitro model (= pseudo\u2010islets). We demonstrate how (pre\u2010) hypoxic \u03b2\u2010cell\u2010composed pseudo\u2010islets can be discriminated from healthy functional pseudo\u2010islets according to their FLIM\u2010based metabolic profiles. The use of FLIM during the pretransplantation pancreatic islet selection process has the potential to improve the outcome of \u03b2\u2010cell islet transplantation.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202000375-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202000375","type":"journal-article","created":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T15:49:10Z","timestamp":1602085750000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Fluorescence lifetime metabolic mapping of hypoxia\u2010induced damage in pancreatic pseudo\u2010islets"],"prefix":"10.1002","volume":"13","author":[{"given":"Aline","family":"Zbinden","sequence":"first","affiliation":[{"name":"Department of Women's Health, Research Institute for Women's Health Eberhard Karls University T\u00fcbingen  T\u00fcbingen 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