{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T12:38:10Z","timestamp":1761395890343,"version":"3.41.2"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,11,25]],"date-time":"2020-11-25T00:00:00Z","timestamp":1606262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The goal of this project is to identify any in\u2010depth benefits and drawbacks in the diagnosis of amalgam tattoos and other pigmented intraoral lesions using hyperspectral imagery collected from amalgam tattoos, benign, and malignant melanocytic neoplasms. Software solutions capable of classifying pigmented lesions of the skin already exist, but conventional red, green and blue images may be reaching an upper limit in their performance. Emerging technologies, such as hyperspectral imaging (HSI) utilize more than a hundred, continuous data channels, while also collecting data in the infrared. A total of 18 paraffin\u2010embedded human tissue specimens of dark pigmented intraoral lesions (including the lip) were analyzed using visible and near\u2010infrared (VIS\u2013NIR) hyperspectral imagery obtained from HE\u2010stained histopathological slides. Transmittance data were collected between 450 and 900\u2009nm using a snapshot camera mounted to a microscope with a halogen light source. VIS\u2013NIR spectra collected from different specimens, such as melanocytic cells and other tissues (eg, epithelium), produced distinct and diagnostic spectra that were used to identify these materials in several regions of interest, making it possible to distinguish between intraoral amalgam tattoos (intramucosal metallic foreign bodies) and melanocytic lesions of the intraoral mucosa and the lip (each with <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.01 using the independent <jats:italic>t<\/jats:italic> test). HSI is presented as a diagnostic tool for the rapidly growing field of digital pathology. In this preliminary study, amalgam tattoos were reliably differentiated from melanocytic lesions of the oral cavity and the lip.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202000424-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202000424","type":"journal-article","created":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T07:32:46Z","timestamp":1605771166000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Hyperspectral imaging as a diagnostic tool to differentiate between amalgam tattoos and other dark pigmented intraoral lesions"],"prefix":"10.1002","volume":"14","author":[{"given":"Johannes","family":"Laimer","sequence":"first","affiliation":[{"name":"University Hospital for Craniomaxillofacial and Oral Surgery, Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuel","family":"Bruckmoser","sequence":"additional","affiliation":[{"name":"Private Practice for Oral and Maxillofacial Surgery  Salzburg Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Helten","sequence":"additional","affiliation":[{"name":"University Hospital for Craniomaxillofacial and Oral Surgery, Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Kofler","sequence":"additional","affiliation":[{"name":"University Hospital of Otorhinolaryngology Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"Zelger","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Neuropathology and Molecular Pathology Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Brunner","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Neuropathology and Molecular Pathology Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernhard","family":"Zelger","sequence":"additional","affiliation":[{"name":"University Hospital for Dermatology, Venereology and Allergology, Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian W.","family":"Huck","sequence":"additional","affiliation":[{"name":"Institute of Analytical Chemistry and Radiochemistry, Leopold Franzens University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Tappert","sequence":"additional","affiliation":[{"name":"Hyperspectral Intelligence Inc.  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Gibsons British Columbia Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Schirmer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Clinic II Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0203-213X","authenticated-orcid":false,"given":"Johannes D.","family":"Pallua","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Neuropathology and Molecular Pathology Medical University of Innsbruck  Innsbruck Austria"},{"name":"University Hospital for Orthopedics and Traumatology Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11912-012-0244-x"},{"key":"e_1_2_9_3_1","unstructured":"A.K.El\u2010Naggar J.K.C.Chan J.R.Grandis T.Takata P.J.Slootweg WHO Classification of Head and Neck Tumours International Agency for Research on Cancer2017."},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2011.11.034"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.23625"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.coc.0000170805.14058.d3"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2008.01.014"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMicm1510216"},{"key":"e_1_2_9_9_1","first-page":"787294","volume":"2013","author":"Lundin K.","year":"2013","journal-title":"Case Rep. 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