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To date, in the US, LD diagnostics is primarily based on validated two\u2010tiered serological testing, which overall exhibits low sensitivity among other drawbacks. In the present study, a potential of Raman spectroscopy (RS) to detect <jats:italic>Bb<\/jats:italic> infection in mice has been explored. For that, C3H mice were infected with wild\u2010type <jats:italic>Bb<\/jats:italic> strains, 297, B31, or B31\u2010derived mutant, \u2206<jats:italic>vlsE<\/jats:italic>. Blood samples taken prior to and post <jats:italic>Bb<\/jats:italic> infection were subjected to RS. The data demonstrated that RS did not directly detect <jats:italic>Bb<\/jats:italic> spirochetes in blood, but rather sensed biochemical changes associated with <jats:italic>Bb<\/jats:italic> infection. Despite <jats:italic>Bb<\/jats:italic> infection\u2010associated blood changes detectable by RS were very limited, the partial least square discriminant analysis showed that the average true positive rates were 86% for 297 and 89% for B31 and \u2206<jats:italic>vlsE<\/jats:italic>.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202000477-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202000477","type":"journal-article","created":{"date-parts":[[2021,1,24]],"date-time":"2021-01-24T13:24:51Z","timestamp":1611494691000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Exploring a possibility of using Raman spectroscopy for detection of Lyme disease"],"prefix":"10.1002","volume":"14","author":[{"given":"Charles","family":"Farber","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biophysics Texas A&amp;M University, College Station  Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohini","family":"Morey","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics Texas A&amp;M University, College Station  Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Krimmer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics Texas A&amp;M University, College Station  Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6040-4213","authenticated-orcid":false,"given":"Dmitry","family":"Kurouski","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics Texas A&amp;M University, College Station  Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6499-7928","authenticated-orcid":false,"given":"Artem S.","family":"Rogovskyy","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University, College Station  Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu397"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2014.940900"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.1998.tb141415.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/07853899909115982"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0b013e3181fc576f"},{"key":"e_1_2_7_7_1","volume":"6","author":"Middelveen M. 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