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In\u2010depth knowledge of target tissue optical properties across a wide\u2010wavelength spectrum could inform the design of optical imaging and computational methods to enable robust tissue analysis during surgery. We adapted a dual\u2010beam integrating sphere to analyse small tissue samples and investigated ex vivo optical properties of five types of human brain tumour (meningioma, pituitary adenoma, schwannoma, low\u2010 and high\u2010grade glioma) and nine different types of healthy brain tissue across a wavelength spectrum of 400 to 1800\u2009nm. Fresh and frozen tissue samples were analysed. All tissue types demonstrated similar absorption spectra, but the reduced scattering coefficients of tumours show visible differences in the obtained optical spectrum compared to those of surrounding normal tissue. 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