{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T04:18:56Z","timestamp":1779164336463,"version":"3.51.4"},"reference-count":70,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T00:00:00Z","timestamp":1624924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mid\u2010infrared (MIR) microscopic imaging of indolent and aggressive lymphomas was performed including formalin\u2010fixed and paraffin\u2010embedded samples of six follicular lymphomas and 12 diffuse large B\u2010cell\u2010lymphomas as well as reactive lymph nodes to investigate benefits and challenges for lymphoma diagnosis. MIR images were compared to defined pathological characteristics such as indolent versus aggressive versus reactive, germinal centre versus activated cell\u2010of\u2010origin (COO) subtypes, or a low versus a high proliferative index and level of PD\u2010L1 expression. We demonstrated that MIR microscopic imaging can differentiate between reactive lymph nodes, indolent and aggressive lymphoma samples. Also, it has potential to be used in the subtyping of lymphomas, as shown with the differentiation between COO subtypes, the level of proliferation and PD\u2010L1 expression. MIR microscopic imaging is a promising tool for diagnosis and subtyping of lymphoma and further evaluation is needed to fully explore the advantages and disadvantages of this method for pathological diagnosis.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202100079-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202100079","type":"journal-article","created":{"date-parts":[[2021,6,23]],"date-time":"2021-06-23T07:31:17Z","timestamp":1624433477000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Application of mid\u2010infrared microscopic imaging for the diagnosis and classification of human lymphomas"],"prefix":"10.1002","volume":"14","author":[{"given":"Ella","family":"Willenbacher","sequence":"first","affiliation":[{"name":"Internal Medicine V: Hematology &amp; Oncology Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Brunner","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Neuropathology and Molecular Pathology, Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"Zelger","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Neuropathology and Molecular Pathology, Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4155-2421","authenticated-orcid":false,"given":"Seraphin H.","family":"Unterberger","sequence":"additional","affiliation":[{"name":"Material\u2010Technology, Leopold\u2010Franzens University Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1066-5340","authenticated-orcid":false,"given":"Roland","family":"Stalder","sequence":"additional","affiliation":[{"name":"Institute of Mineralogy and Petrography, Leopold\u2010Franzens University Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6272-3242","authenticated-orcid":false,"given":"Christian W.","family":"Huck","sequence":"additional","affiliation":[{"name":"Institute of Analytical Chemistry and Radiochemistry  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Willenbacher","sequence":"additional","affiliation":[{"name":"Internal Medicine V: Hematology &amp; Oncology Medical University of Innsbruck  Innsbruck Austria"},{"name":"Oncotyrol, Center for personalized Cancer Medicine  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0203-213X","authenticated-orcid":false,"given":"Johannes D.","family":"Pallua","sequence":"additional","affiliation":[{"name":"Institute of Pathology, Neuropathology and Molecular Pathology, Medical University of Innsbruck  Innsbruck Austria"},{"name":"University Hospital for Orthopedics and Traumatology, Medical University of Innsbruck  Innsbruck Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,6,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.2006.00775.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2an35483g"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.037986"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.05.015"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1364\/AO.44.003725"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1997.4627"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.26.15327"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6343(1998)4:1<47::AID-BSPY5>3.0.CO;2-P"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6343(1998)4:1<55::AID-BSPY6>3.0.CO;2-R"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2003.12.028"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707567104"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4872"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(96)04450-3"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-006-0827-1"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1421"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0mb00041h"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1080"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-011-5123-z"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20154"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.20.8140"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2003.12.006"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6343(1999)5:4<219::AID-BSPY2>3.0.CO;2-O"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5AN01072A"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.20492"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-4116-7"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-008-2443-8"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-008-2592-9"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-2737-5"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6343(1998)4:2<75::AID-BSPY1>3.0.CO;2-R"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.104.053926"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0282(2000)57:6<329::AID-BIP20>3.0.CO;2-2"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(03)74621-9"},{"key":"e_1_2_6_34_1","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow S. H.","year":"2017"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajhp\/zxz202"},{"issue":"2","key":"e_1_2_6_36_1","first-page":"435","volume":"6","author":"Senevirathna K.","year":"2021","journal-title":"Med. Clin. Res."},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2014-0451-CP"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-05-1545"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.21406"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-14-153"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-004-0855-x"},{"key":"e_1_2_6_42_1","first-page":"1113","volume":"28","author":"Szczuraszek K.","year":"2008","journal-title":"Anticancer Res."},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.2914"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018020602"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1039\/C1AN15615B"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470988541"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470283172"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527628230"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-2794-9"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1039\/b715924b"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1366\/0003702944028308"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05573"},{"key":"e_1_2_6_53_1","first-page":"189","volume":"44","author":"Lasch P.","year":"1998","journal-title":"Cell. Mol. Biol. (Noisy\u2010le\u2010Grand)"},{"key":"e_1_2_6_54_1","first-page":"1270","volume":"30","author":"Wei P. Y.","year":"2007","journal-title":"Zhong Yao Cai"},{"key":"e_1_2_6_55_1","first-page":"203","volume":"44","author":"Schultz C. P.","year":"1998","journal-title":"Cell. Mol. Biol. (Noisy\u2010le\u2010Grand)"},{"key":"e_1_2_6_56_1","first-page":"89","volume":"44","author":"Jackson M.","year":"1998","journal-title":"Cell. Mol. Biol. (Noisy\u2010le\u2010Grand)"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0282(2000)57:5<282::AID-BIP50>3.0.CO;2-R"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1366\/000370203321165151"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-6111(97)00018-9"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470061190"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vibspec.2005.10.005"},{"key":"e_1_2_6_62_1","volume-title":"Biomedical Vibrational Spectroscopy","author":"Lasch P.","year":"2007"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2008.08.014"},{"key":"e_1_2_6_64_1","volume-title":"Techniques and Applications of Hyperspectral Image Analysis","author":"Geladi P.","year":"2007"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0174552"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7AY01132F"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.26771"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-02-629600"},{"key":"e_1_2_6_69_1","first-page":"2393","volume":"19","author":"Svoboda J.","year":"2020","journal-title":"Haematologica"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.pas.0000172191.87176.3b"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001609"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202100079","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbio.202100079","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202100079","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T18:28:34Z","timestamp":1693333714000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.202100079"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,29]]},"references-count":70,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1002\/jbio.202100079"],"URL":"https:\/\/doi.org\/10.1002\/jbio.202100079","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,29]]},"assertion":[{"value":"2021-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202100079"}}