{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:30:30Z","timestamp":1788395430990,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T00:00:00Z","timestamp":1637020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The biological relevance of nitric oxide (NO) and reactive oxygen species (ROS) in signaling, metabolic regulation, and disease treatment has become abundantly clear. The dramatic change in NO\/ROS processing that accompanies a changing oxygen landscape calls for new imaging tools that can provide cellular details about both [O<jats:sub>2<\/jats:sub>] and the production of reactive species. Myoglobin oxidation to the <jats:italic>met<\/jats:italic> state by NO\/ROS is a known sensor with absorbance changes in the visible range. We previously employed F\u00f6rster resonance energy transfer to read out the deoxygenation\/oxygenation of myoglobin, creating the subcellular [O<jats:sub>2<\/jats:sub>] sensor Myoglobin\u2010mCherry. We now add the fluorescent protein EYFP to this sensor to create a novel probe that senses both <jats:italic>met<\/jats:italic> formation, a proxy for ROS\/NO exposure, and [O<jats:sub>2<\/jats:sub>]. Since both proteins are present in the construct, it can also relieve users from the need to measure fluorescence lifetime, making [O<jats:sub>2<\/jats:sub>] sensing available to a wider group of laboratories.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202100166-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202100166","type":"journal-article","created":{"date-parts":[[2021,10,24]],"date-time":"2021-10-24T04:04:00Z","timestamp":1635048240000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Intracellular <i>imaging<\/i> of metmyoglobin and oxygen using new dual purpose probe <scp>EYFP\u2010Myoglobin\u2010mCherry<\/scp>"],"prefix":"10.1002","volume":"15","author":[{"given":"Rozhin","family":"Penjweini","sequence":"first","affiliation":[{"name":"Laboratory of Advanced Microscopy and Biophotonics National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Branden","family":"Roarke","sequence":"additional","affiliation":[{"name":"Laboratory of Advanced Microscopy and Biophotonics National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg","family":"Alspaugh","sequence":"additional","affiliation":[{"name":"Laboratory of Advanced Microscopy and Biophotonics National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie A.","family":"Link","sequence":"additional","affiliation":[{"name":"Laboratory of Advanced Microscopy and Biophotonics National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessio","family":"Andreoni","sequence":"additional","affiliation":[{"name":"Laboratory of Advanced Microscopy and Biophotonics National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health (NIH)  Bethesda Maryland USA"},{"name":"Laboratory of Optical Neurophysiology, Department of Biochemistry and Molecular Medicine University of California Davis  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus P.","family":"Mori","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular and Cancer Genetics National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul M.","family":"Hwang","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular and Cancer Genetics National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan L.","family":"Sackett","sequence":"additional","affiliation":[{"name":"Cytoskeletal Dynamics Group, Division of Basic and Translational Biophysics Eunice Kennedy Shriver National Institute of Child Health and Human Development, NIH  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9990-1738","authenticated-orcid":false,"given":"Jay R.","family":"Knutson","sequence":"additional","affiliation":[{"name":"Laboratory of Advanced Microscopy and Biophotonics National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00036.2017"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000260171.52224.6b"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2018.00006"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.02265-06"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI133181"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.92"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101549"},{"key":"e_1_2_9_9_1","first-page":"1","volume":"23","author":"Penjweini R.","year":"2018","journal-title":"J. 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