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In the past, we used the combination of second harmonic generation and fast Fourier transform to study a number of topics including the structural alteration of human keratoconus. Recently, we applied this approach to studying changes of chick embryonic cornea during development. As numerous studies were performed with <jats:italic>ex vivo<\/jats:italic> specimens, a thorough understanding of the parameters associated with the development process cannot be achieved until experiments are performed in living chick embryos.<\/jats:p>","DOI":"10.1002\/jbio.202100210","type":"journal-article","created":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T07:14:45Z","timestamp":1643181285000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dynamic development of the extracellular matrix in chick cornea"],"prefix":"10.1002","volume":"15","author":[{"given":"Yang\u2010Fang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Physics National Taiwan University  Taipei Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4487-2965","authenticated-orcid":false,"given":"Chen\u2010Yuan","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Physics National Taiwan University  Taipei Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201900144"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.08.017"},{"key":"e_1_2_6_4_1","unstructured":"J Cell Biol 1971 50"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21579"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1030510102"},{"key":"e_1_2_6_7_1","first-page":"405","volume-title":"The Development of the Structural and Optical Properties of the Cornea, The Structure of the Eye","author":"Coulombre A. 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