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In this study, a single\u2010cell\u2010layer microfluidic device was developed. After replacing the optical clearing agent, the characteristics of H8, HeLa and SiHa cell lines in adherent and suspended states were detected. Additionally, the absorption increased with increasing cell density. For the mixed suspension cell samples, principal component analysis\u2013support vector machine method was used to identify benign and malignant cell component. After living cells formaldehyde, changes in cell membrane permeability were evaluated to identify the cell survival status (i.e., dead or living) based on terahertz spectroscopy amplitude differences. Therefore, extending the terahertz spectrum detection to the molecular level can characterize the life essence of cells and tissues.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202100241-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202100241","type":"journal-article","created":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T10:38:37Z","timestamp":1635331117000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Label\u2010free detection of living cervical cells based on microfluidic device with terahertz spectroscopy"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9331-0512","authenticated-orcid":false,"given":"Xiaoyue","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Affiliated Hospital of Soochow University  Suzhou 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