{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T08:09:58Z","timestamp":1768637398640,"version":"3.49.0"},"reference-count":85,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T00:00:00Z","timestamp":1657238400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["\u211619\u201075\u201010084"],"award-info":[{"award-number":["\u211619\u201075\u201010084"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this article, we offer a novel classification of progressive changes in the connective tissue of dermis in vulvar lichen sclerosus (VLS) relying on quantitative assessment of the second harmonic generation (SHG) signal received from formalin fixed and deparaffinized tissue sections. We formulate criteria for distinguishing four degrees of VLS development: Initial\u2010Mild\u2010Moderate\u2010Severe. Five quantitative characteristics (length and thickness type I Collagen fibers, Mean SHG signal intensity, Skewness and Coherence SHG signal) are used to describe the sequential degradation of connective tissue (changes in the structure, orientation, shape and density of collagen fibers) up to the formation of specific homogeneous masses. Each of the degrees has a characteristic set of quantitatively expressed features. We focus on the identification and description of early, initial changes of the dermis as the least specific. The results obtained by us and the proposed classification of the degrees of the disease can be used to objectify the dynamics of tissue changes during treatment.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202200036-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202200036","type":"journal-article","created":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T14:39:32Z","timestamp":1654180772000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Multiphoton microscopy assessment of the structure and variability changes of dermal connective tissue in vulvar lichen sclerosus: A pilot study"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4343-2500","authenticated-orcid":false,"given":"Arseniy L.","family":"Potapov","sequence":"first","affiliation":[{"name":"Privolzhsky Research Medical University  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina A.","family":"Sirotkina","sequence":"additional","affiliation":[{"name":"Privolzhsky Research Medical University  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lev A.","family":"Matveev","sequence":"additional","affiliation":[{"name":"Institute of Applied Physics Russian Academy of Sciences  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Varvara V.","family":"Dudenkova","sequence":"additional","affiliation":[{"name":"Privolzhsky Research Medical University  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vadim V.","family":"Elagin","sequence":"additional","affiliation":[{"name":"Privolzhsky Research Medical University  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey S.","family":"Kuznetsov","sequence":"additional","affiliation":[{"name":"N.A. Semashko Nizhny Novgorod Regional Clinical Hospital  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria M.","family":"Karabut","sequence":"additional","affiliation":[{"name":"Privolzhsky Research Medical University  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia D.","family":"Komarova","sequence":"additional","affiliation":[{"name":"Privolzhsky Research Medical University  Nizhny Novgorod Russia"},{"name":"Lobachevsky State University of Nizhny Novgorod  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nailya N.","family":"Vagapova","sequence":"additional","affiliation":[{"name":"N.A. Semashko Nizhny Novgorod Regional Clinical Hospital  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan K.","family":"Safonov","sequence":"additional","affiliation":[{"name":"Privolzhsky Research Medical University  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina A.","family":"Kuznetsova","sequence":"additional","affiliation":[{"name":"N.A. Semashko Nizhny Novgorod Regional Clinical Hospital  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefka G.","family":"Radenska\u2010Lopovok","sequence":"additional","affiliation":[{"name":"I.M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena V.","family":"Zagaynova","sequence":"additional","affiliation":[{"name":"Privolzhsky Research Medical University  Nizhny Novgorod Russia"},{"name":"Lobachevsky State University of Nizhny Novgorod  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia D.","family":"Gladkova","sequence":"additional","affiliation":[{"name":"Privolzhsky Research Medical University  Nizhny Novgorod Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gore.2017.03.003"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJWH.S191200"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00040.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.34613"},{"key":"e_1_2_9_6_1","first-page":"2559","volume":"16","author":"Zhao Y.","year":"2018","journal-title":"Oncol Lett."},{"key":"e_1_2_9_7_1","volume-title":"Robboy's Pathology of the Female Reproductive Tract","author":"Robboy S. J.","year":"2008"},{"key":"e_1_2_9_8_1","first-page":"844","volume":"11","author":"Carlson J. A.","year":"1998","journal-title":"Mod. Pathol."},{"issue":"181","key":"e_1_2_9_9_1","first-page":"e181","volume":"202","author":"Kauppila S.","year":"2010","journal-title":"Am. J. Obstet. Gynecol."},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-1807-2"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0b013e31827e7ea9"},{"key":"e_1_2_9_12_1","first-page":"781","volume":"31","author":"Hewitt J.","year":"1986","journal-title":"J. Reprod. Med."},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.13136"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1997.6061582.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2005.02209.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.17447"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.2.002307"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5920"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.65.109"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1047-8477(02)00576-2"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9101(2000)27:4<329::AID-LSM5>3.0.CO;2-C"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7113-8_27"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.02.059"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0846.2009.00381.x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1998.tb02521.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/sca.20219"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.172368799"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00192-014-2571-y"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.200910062"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370220934039"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1117\/12.382720"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0520969"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.14.007810"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.30.002275"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.200710003"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202100055"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1117\/12.2555366"},{"key":"e_1_2_9_38_1","doi-asserted-by":"crossref","unstructured":"M. A.Sirotkina A. L.Potapov E. A.Konovalova N. N.Vagapova I. K.Safonov A. A.Timakova S. G.Radenska\u2010Lopovok I. A.Kuznetsova D. A.Karashtin L. A. Matveev in Optical coherence tomography for complex diagnosis of vulvar diseases 11228 International Society for Optics and Photonics City 2020 pp.1122815.","DOI":"10.1117\/12.2545801"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0000000000000162"},{"key":"e_1_2_9_40_1","first-page":"8","volume-title":"\"Three Rings Vulvoscopy\" a New Approach to the Vulva, Naprijed, Zagreb, Croatia","author":"Harni V.","year":"2016"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00371568"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00105-003-0645-8"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2004.01929.x"},{"key":"e_1_2_9_44_1","volume-title":"Pathology of the Female Reproductive Tract","author":"George M.","year":"2014"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1940.01490170003001"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.085902"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.097998"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)90878-N"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1106"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2009.12.009"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147804"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep02190"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10237-011-0325-z"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gie.2010.12.016"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.108.528091"},{"key":"e_1_2_9_57_1","first-page":"2011.03065","author":"Tian Q.","year":"2020","journal-title":"arXiv preprint"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.1997.tb00481.x"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.13741"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.12342"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2010.11.020"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-012-0512-9"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.6061\/clinics\/2015(05)09"},{"key":"e_1_2_9_64_1","first-page":"38","volume":"52","author":"Corr\u00eaa A. C.","year":"2007","journal-title":"J. Reprod. Med."},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.839786"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1117\/12.2505859"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2009.03146.x"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2015.0643"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.140.6.702"},{"key":"e_1_2_9_70_1","volume-title":"Diagnostic Aspects of Lichen Sclerosus and Skin Cancer Studied with Laser Scanning Microscopy","author":"Carlsson A.","year":"2020"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2014.07.008"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/srt.12460"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/srt.12246"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.200810045"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.1994.tb01183.x"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.25.1.014515"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1117\/12.702407"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00402628"},{"key":"e_1_2_9_79_1","first-page":"3","volume":"162","author":"Oikarinen A.","year":"1991","journal-title":"Acta Derm Venereol Suppl (Stockh)."},{"key":"e_1_2_9_80_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.2340\/00015555721518","volume":"72","author":"Griffiths M. R.","year":"1992","journal-title":"Acta Derm. Venereol."},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-016-0204-7"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0216537"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0180751"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.95.4.649"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23330"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2012.02.017"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202200036","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbio.202200036","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202200036","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T08:08:56Z","timestamp":1692778136000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.202200036"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,8]]},"references-count":85,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1002\/jbio.202200036"],"URL":"https:\/\/doi.org\/10.1002\/jbio.202200036","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,8]]},"assertion":[{"value":"2022-02-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202200036"}}