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And the TMJ health index was invented. TMJ pathologies are commonly and reported frequently. It could be associated with the damage of collagen, cartilage and bone tissue. The second harmonic generation images could be obtained by a femtosecond laser pulses, so the aligned information of the collagen fibers in all directions for the TMJ disorders was collected. The disorder degree of collagen fibers was quantified and ranked using a fast Fourier transform (FFT) method. The TMJ health index can effectively present the TMJ healthy condition and the disorder degree of collagen fibers, a valuable objective tool for tissue characterization for TMJ healthy condition. Integrated with the staining methods, we can provide the scaling information at different injury degree.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202200075-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202200075","type":"journal-article","created":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T15:58:02Z","timestamp":1652975882000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Diagnosing temporomandibular joint disorders using second harmonic imaging of collagen fibers"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2750-0780","authenticated-orcid":false,"given":"Yang","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Physics Dalian University of Technology  Dalian Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7971-7180","authenticated-orcid":false,"given":"Jing\u2010Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of 3D Printing Technology in Stomatology The First Dongguan Affiliated Hospital Guangdong Medical University  Dongguan Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Optoelectronic Engineering and Instrumentation Science Dalian University of Technology  Dalian Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsiang\u2010Chen","family":"Chui","sequence":"additional","affiliation":[{"name":"School of Optoelectronic Engineering and Instrumentation Science Dalian University of Technology  Dalian Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiajia","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research Xi'an Jiaotong University  Xi'an Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0104-9386","authenticated-orcid":false,"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Physics Dalian University of Technology  Dalian Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,7,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-018-0462-8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","unstructured":"SGomes \u00c2David\u2010Faustino PFPaula AMateus NAlves Finite element analysis of a human temporomandibular joint disc Preliminary results. 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