{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T15:56:14Z","timestamp":1782489374018,"version":"3.54.5"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T00:00:00Z","timestamp":1664841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["63223052"],"award-info":[{"award-number":["63223052"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["63221010"],"award-info":[{"award-number":["63221010"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["12104241"],"award-info":[{"award-number":["12104241"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Myopia and keratoconus have become common corneal diseases that threaten the quality of human vision, and keratoconus is one of the most common indications for corneal transplantation worldwide. Collagen crosslinking (CXL) using riboflavin and ultraviolet A (UVA) light is an effective approach for treating ophthalmic disorders and has been shown clinically not only to arrest further progression of keratoconus but also to improve refractive power for cornea. However, CXL surgery irradiated by UVA has various potential risks such as surface damage and endothelial cell damage. Here, near\u2010infrared femtosecond laser\u2010based two\u2010photon CXL was first applied to ex vivo human corneal stroma, operating at low photon energy with high precision and stability. After two\u2010photon CXL, the corneal stiffness can be enhanced by 300% without significantly reducing corneal transparency. These findings illustrate the optimized direction that depositing high pulses energy in corneal focal volume (not exceeding damage threshold), and pave the way to 3D CXL of in vivo human cornea with higher safety, precision, and efficacy.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202200160-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202200160","type":"journal-article","created":{"date-parts":[[2022,9,25]],"date-time":"2022-09-25T02:53:02Z","timestamp":1664074382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Two\u2010photon collagen crosslinking in ex vivo human corneal lenticules induced by near\u2010infrared femtosecond laser"],"prefix":"10.1002","volume":"16","author":[{"given":"Zhenzhou","family":"Cheng","sequence":"first","affiliation":[{"name":"Institute of Modern Optics, Eye Institute Nankai University, Key Laboratory of Micro\u2010scale Optical Information Science and Technology Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Modern Optics, Eye Institute Nankai University, Key Laboratory of Micro\u2010scale Optical Information Science and Technology Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Chang","sequence":"additional","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Laboratory of Ophthalmology and Visual Science Tianjin China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2885-2072","authenticated-orcid":false,"given":"Pengfei","family":"Qi","sequence":"additional","affiliation":[{"name":"Institute of Modern Optics, Eye Institute Nankai University, Key Laboratory of Micro\u2010scale Optical Information Science and Technology Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Laboratory of Ophthalmology and Visual Science Tianjin China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lie","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Modern Optics, Eye Institute Nankai University, Key Laboratory of Micro\u2010scale Optical Information Science and Technology Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Modern Optics, Eye Institute Nankai University, Key Laboratory of Micro\u2010scale Optical Information Science and Technology Tianjin China"},{"name":"Tianjin Eye Hospital Tianjin Key Laboratory of Ophthalmology and Visual Science Tianjin China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6036-9641","authenticated-orcid":false,"given":"Weiwei","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Modern Optics, Eye Institute Nankai University, Key Laboratory of Micro\u2010scale Optical Information Science and Technology Tianjin China"},{"name":"Tianjin Eye Hospital Tianjin Key Laboratory of Ophthalmology and Visual Science Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/519276a"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2016.01.006"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18-25957"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2020.03.006"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9071.2011.02681.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/cxo.12211"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.optom.2014.05.006"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2017.03.036"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","unstructured":"Y. Z.Guo C.Wang N.Celi S.Vukelic Optical Interactions with Tissue and Cells Xxvi 2015 9321.","DOI":"10.1117\/12.2079608"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.19.015996"},{"key":"e_1_2_7_12_1","unstructured":"C.Wang K.Durney M.Fomovsky J.Yu J.Roland\u2010Hall G.Ateshian S.Vukeli\u0107 presented atAnnual Meeting of the Orthopaedic Research Society. San Diego CA USA 2017."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41566-018-0174-8"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1997.0410"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(02)02220-1"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.icu.0000233954.86723.25"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2013.01.002"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1167\/tvst.10.5.4"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2018.01.022"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijo.IJO_1227_17"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0104(83)85070-8"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.11-9385"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2007.12.039"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2009.10.021"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200401000-00008"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e3180334f78"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.10-7105"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1586\/eop.10.7"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2011.05.036"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2005.12.092"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00347-007-1587-9"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.2007.01142.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-56529-1"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.5301\/JABFM.2012.9278"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.2741\/E642"},{"key":"e_1_2_7_36_1","first-page":"399","volume":"58","author":"Koroleva A.","year":"2013","journal-title":"Biomed. Eng. Biomed. Tech."},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme.13.60"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709571105"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/adom.202000584"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.37188\/lam.2021.003"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abj2691"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2016.06.040"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.8.004788"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1167\/tvst.8.6.35"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.18.3.038003"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/opo.12345"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.082388"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2321027"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.17-22002"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-46572-3"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.elecom.2006.01.026"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrs.2008.05.033"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)33209-4"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.387826"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201800472"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0886-3350(03)00407-3"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/406850"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2020.108199"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.16-20241"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2010.06.041"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202200160","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbio.202200160","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202200160","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T15:52:22Z","timestamp":1728057142000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.202200160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,4]]},"references-count":59,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1002\/jbio.202200160"],"URL":"https:\/\/doi.org\/10.1002\/jbio.202200160","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,4]]},"assertion":[{"value":"2022-05-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-09-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202200160"}}