{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T03:42:47Z","timestamp":1769571767178,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T00:00:00Z","timestamp":1684281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["12027808"],"award-info":[{"award-number":["12027808"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["11834008"],"award-info":[{"award-number":["11834008"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["11874217"],"award-info":[{"award-number":["11874217"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971629"],"award-info":[{"award-number":["81971629"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microcirculation imaging has significantly clinical value in early diagnosis and curative effect judgment of various diseases. The most superficial layer of the nailbed is rich in capillaries, which is suitable as a window on the microcirculation. However, few techniques can noninvasively observe the blood supply distribution of the nailbed, especially for high\u2010resolution imaging of capillaries. In this study, we adapted an optical\u2010resolution photoacoustic microscopy (OR\u2010PAM) to image the nailbed microvasculature. The imaging sensitivity was significantly improved by hydration pretreatment of the nail. In vitro phantom experiments demonstrate that the sensitivity was improved about 3.5 times after hydration. In vivo imaging experiments of the nailbed microvasculature were conducted to further examine the enhanced sensitivity and practicability of OR\u2010PAM. Moreover, the quantitative analysis of capillary loops showed that OR\u2010PAM can extract the detection indicators including vascular morphology, diameter, and length, which provides a basis for clinical microcirculation detection using OR\u2010PAM.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202300058-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202300058","type":"journal-article","created":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T05:50:56Z","timestamp":1683870656000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["High\u2010resolution in vivo imaging of human nailbed microvasculature by using photoacoustic microscopy"],"prefix":"10.1002","volume":"16","author":[{"given":"Dongfang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Physics, Collaborative Innovation Center of Advanced Microstructures, MOE Key Laboratory of Modern Acoustics Nanjing University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manli","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Physics, Collaborative Innovation Center of Advanced Microstructures, MOE Key Laboratory of Modern Acoustics Nanjing University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8052-6827","authenticated-orcid":false,"given":"Chao","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Physics, Collaborative Innovation Center of Advanced Microstructures, MOE Key Laboratory of Modern Acoustics Nanjing University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqin","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Ultrasound Affiliated People's Hospital of Jiangsu University  Zhenjiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaojun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physics, Collaborative Innovation Center of Advanced Microstructures, MOE Key Laboratory of Modern Acoustics Nanjing University  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201800421"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202000392"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202200164"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.430403"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.434004"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep06525"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.35.001419"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-010-2005-3"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10877-012-9383-8"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0b013e3181dde28d"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0000000000000332"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1549-8719.2012.00188.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.763730"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2002.01714.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2015.04.004"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3925"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.22.4.041002"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.5143155"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.21.5.056004"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.42.004434"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.43.001615"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.388863"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhsu.2001.21519"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2018.04.017"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1117\/12.2527050"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-020-02400-y"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.65.061913"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-014-1562-5"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1989.01670170057008"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.22.001094"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pacs.2021.100313"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/photonics8040127"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1088\/0031-9155\/49\/7\/018"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.023218"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11249-010-9616-2"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2013.06.003"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.130528"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.150900"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202300058","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T10:53:29Z","timestamp":1692269609000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.202300058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,17]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["10.1002\/jbio.202300058"],"URL":"https:\/\/doi.org\/10.1002\/jbio.202300058","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,17]]},"assertion":[{"value":"2023-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202300058"}}