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Rhodamine (Rho) labeled bovine serum albumin (BSA) and chitosan (Chi) were prepared by covalent conjugation and were characterized by fluorescence spectral analysis. GNP and SNP were coated with the labeled conjugates of BSA and chitosan by adsorption. The soluble Rho\u2010BSA or Rho\u2010Chi conjugates, uncoated, and conjugate\u2010coated GNP, and SNP were orally administered into 8\u2010week\u2010old rats. After 24\u2009h, rats were euthanized and the liver, kidney, spleen, and thymus were dissected. The tissues were examined ex vivo using a small animal in vivo imaging system. The liver, kidney, and thymus displayed higher fluorescence due to increased accumulation of Rho\u2010BSA or Rho\u2010Chi conjugate\u2010coated nanoparticles (NPs) in the tissues as compared to the spleen where lower fluorescence was noticed. Tissues obtained from rats that were administered Rho\u2010BSA or Rho\u2010Chi conjugate\u2010coated GNP and SNP showed tenfold higher fluorescence intensity as compared to tissues from rats that were given soluble conjugates or NP alone. The results strongly suggest significant tissue distribution of NP following oral administration.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202300110-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202300110","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T14:49:56Z","timestamp":1685630996000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Ex vivo fluorescence imaging for the identification of rhodamine\u2010labeled bovine serum albumin and chitosan\u2010coated gold and silver nanoparticles"],"prefix":"10.1002","volume":"16","author":[{"given":"Mallaiah","family":"Devanabanda","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Bioinformatics Yogi Vemana University  Kadapa Andhra Pradesh India"},{"name":"Cellular Immunology Laboratory, Department of Biochemistry, School of Life 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