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Since the sample arm of the interferometer is comprised of multiple glass materials, the ensuing dispersion severely degrades imaging quality. In this article, second\u2010order dispersion simulation analysis for various materials was performed first, and dispersion equilibrium was implemented utilizing physical compensation methods. After dispersion compensation, an imaging depth in air of 4.013\u2009mm was achieved in model eye experiments, and signal\u2010to\u2010noise ratio was enhanced by 11.6%, with a value of 53.8\u2009dB. In vivo imaging of the human retina was performed to demonstrate structurally distinguishable retinal images, characterized by an axial resolution improvement of 19.8%, with a value of 7.7\u2009\u03bcm close to the theoretical value of 7.5\u2009\u03bcm. The proposed physical dispersion compensation method enhances imaging performance in SS\u2010OCT systems, enabling visualization of several low scattering mediums.\n                    <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202300167-gra-0001.png\" xlink:title=\"image\"\/>\n                  <\/jats:p>","DOI":"10.1002\/jbio.202300167","type":"journal-article","created":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T09:19:34Z","timestamp":1687943974000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Physical compensation method for dispersion of multiple materials in swept source optical coherence tomography"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6317-6132","authenticated-orcid":false,"given":"Jiangjie","family":"Huang","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Jiangsu Key Laboratory of Medical Optics Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyu","family":"Fan","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Jiangsu Key Laboratory of Medical Optics Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"He","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Jiangsu Key Laboratory of Medical Optics Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohua","family":"Shi","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Jiangsu Key Laboratory of Medical Optics Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences  Suzhou China"},{"name":"Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences  Shanghai China"},{"name":"Key Laboratory of Myopia of State Health Ministry Key Laboratory of Visual Impairment and Restoration of Shanghai  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1957169"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/20406223231170146"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2021-320131"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.393906"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.28.002067"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.11.002183"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.9.006569"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20404-9"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-04784-0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1364\/OPEX.13.003252"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.3119103"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.17.024304"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-56847-4"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1142\/S1793545809000383"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.429900"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-48173-7"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.24.001221"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.20.001486"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1364\/OPEX.12.005968"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11082-005-4192-7"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1364\/OPEX.12.002404"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.9.000610"},{"key":"e_1_2_8_24_1","volume-title":"Optical Coherence Tomography: Technology and Applications","author":"Bouma B. 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G.Schott.Optical glass collection datasheets (2017).https:\/\/www.schott.com\/en\u2010us\/products\/optical\u2010glass\u2010p1000267\/downloads."},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1364\/JOSA.55.001205"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1997.0401"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1364\/AO.37.005679"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202300167","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbio.202300167","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202300167","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T06:18:08Z","timestamp":1790921888000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.202300167"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,7]]},"references-count":29,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.1002\/jbio.202300167"],"URL":"https:\/\/doi.org\/10.1002\/jbio.202300167","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,7]]},"assertion":[{"value":"2023-05-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-07-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202300167"}}