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Despite the efficient ablation of the melanophores in the skin, the single, high\u2010fluence pulse often causes undesirable damage to the surrounding tissue, leading to high recurrence rates. This study aims to investigate the feasibility of dual\u2010optical pulses with a temporal energy distribution on the melasma treatment in in vivo zebrafish models in comparison to that of the single optical pulse. Based on the optical detection, the dual\u2010optical pulses had a temporal energy distribution ratio of 4:1 and an interval of 61\u2009\u03bcs between the two consecutive pulses. According to the histological analysis, the dual pulses removed melanophores and induced a few apoptotic nuclei with minimal recurrence. This study demonstrated that the feasibility of dual\u2010optical pulses (energy ratio\u2009=\u20094:1) could enhance the laser ablation performance in vivo.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202300232-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202300232","type":"journal-article","created":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T19:04:40Z","timestamp":1693508680000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of dual\u2010optical pulses with temporal energy distribution on laser ablation performance in in vivo zebrafish model"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7440-3780","authenticated-orcid":false,"given":"Hwarang","family":"Shin","sequence":"first","affiliation":[{"name":"Industry 4.0 Convergence Bionics Engineering Pukyong National University  Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6068-5409","authenticated-orcid":false,"given":"Yeachan","family":"Lee","sequence":"additional","affiliation":[{"name":"Marine\u2014Integrated Biomedical Technology Center Pukyong National University  Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6370-1184","authenticated-orcid":false,"given":"Junghyun","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Division of Smart Healthcare, College of Information Pukyong National University  Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9103-3550","authenticated-orcid":false,"given":"Seonghee","family":"Lim","sequence":"additional","affiliation":[{"name":"Industry 4.0 Convergence Bionics Engineering Pukyong National University  Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9295-7111","authenticated-orcid":false,"given":"Jongman","family":"Choi","sequence":"additional","affiliation":[{"name":"Research and Development Bluecore Company  Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9358-9568","authenticated-orcid":false,"given":"Seung Pyo","family":"Gong","sequence":"additional","affiliation":[{"name":"Division of Fisheries Life Science, Major in Aquaculture and Applied Life Science Pukyong National University  Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0861-1354","authenticated-orcid":false,"given":"Hyun Wook","family":"Kang","sequence":"additional","affiliation":[{"name":"Industry 4.0 Convergence Bionics Engineering Pukyong National University  Busan Republic of Korea"},{"name":"Marine\u2014Integrated Biomedical Technology Center Pukyong National University  Busan Republic of Korea"},{"name":"Department of Biomedical Engineering, Division of Smart Healthcare, College of Information Pukyong National University  Busan Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,9,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina58070936"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.23474"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/09546634.2021.1914313"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0974-2077.99436"},{"key":"e_1_2_10_6_1","first-page":"4136","volume":"18","author":"Guo X.","year":"2019","journal-title":"Oncol. 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