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PDT induces apoptosis and necrosis in AKs and cSCC. 5\u2010ALA blue light PDT may modulate gene expression and pathways in surviving cells. In this study, differential gene expression and pathway analysis of cSCC and human dermal fibroblasts were compared before and after 5\u2010ALA blue light PDT using RNA sequencing. No genes were differentially expressed after correcting for multiple testing (false discovery rate\u2009&lt;\u20090.05). As a result, transcription factor, gene enrichment, and pathway analysis were performed with genes identified before multiple testing (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Pathways associated with proliferation and carcinogenesis were downregulated. These findings using 5\u2010ALA blue light PDT are similar to previously published studies using methyl\u2010aminolevulinic and red light protocols, indicating that surviving residual cells may undergo changes consistent with a less aggressive cancerous phenotype.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202300369-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202300369","type":"journal-article","created":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T06:10:26Z","timestamp":1707459026000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Blue light aminolevulinic acid photodynamic therapy downregulates cell division and proliferation pathways in cutaneous squamous cell carcinoma"],"prefix":"10.1002","volume":"17","author":[{"given":"Evan","family":"Austin","sequence":"first","affiliation":[{"name":"Department of Dermatology SUNY Downstate Medical Center  Brooklyn New York USA"},{"name":"Department of Dermatology VA New York Harbor Healthcare System  Brooklyn New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Mineroff","sequence":"additional","affiliation":[{"name":"Department of Dermatology SUNY Downstate Medical Center  Brooklyn New York USA"},{"name":"Department of Dermatology VA New York Harbor Healthcare System  Brooklyn New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabella","family":"Dana","sequence":"additional","affiliation":[{"name":"Department of Dermatology SUNY Downstate Medical Center  Brooklyn New York USA"},{"name":"Department of Dermatology VA New York Harbor Healthcare System  Brooklyn New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7619-7650","authenticated-orcid":false,"given":"Jared","family":"Jagdeo","sequence":"additional","affiliation":[{"name":"Department of Dermatology SUNY Downstate Medical Center  Brooklyn New York USA"},{"name":"Department of Dermatology VA New York Harbor Healthcare System  Brooklyn New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.5826\/dpc.1003a66"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/DSS.0000000000000478"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700897"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/archdermatol.2010.19"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.4046"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abd.2019.10.004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2000.103342"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amepre.2014.08.036"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1684\/ejd.2015.2570"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers8090083"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.16017"},{"key":"e_1_2_7_13_1","first-page":"E53","volume":"14","author":"Zeitouni N. 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