{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T09:33:50Z","timestamp":1769074430617,"version":"3.49.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T00:00:00Z","timestamp":1702598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P41EB031772"],"award-info":[{"award-number":["P41EB031772"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01CA241618"],"award-info":[{"award-number":["R01CA241618"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01EB023232"],"award-info":[{"award-number":["R01EB023232"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01CA213149"],"award-info":[{"award-number":["R01CA213149"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01EB028615"],"award-info":[{"award-number":["R01EB028615"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["CBET 18\u201041539"],"award-info":[{"award-number":["CBET 18\u201041539"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> (<jats:italic>P. aeruginosa<\/jats:italic>) is a multidrug\u2010resistant human pathogen involved in numerous infections. Understanding the response of <jats:italic>P. aeruginosa<\/jats:italic> to various treatments is critical to developing new ways for the antimicrobial susceptibly test and more effective treatment methods. Conventional antimicrobial susceptibility tests lack molecular information at the single bacterium level. In this study, we used label\u2010free multimodal nonlinear optical microscopy to identify an autofluorescence signal from pyoverdine, a siderophore of the bacteria, for quantification of <jats:italic>P. aeruginosa<\/jats:italic> responses to antibiotics and blue light treatment. We also discovered that the bleaching of the pyoverdine autofluorescence signals is correlated with the inactivation of <jats:italic>P. aeruginosa<\/jats:italic> and is perhaps one of the mechanisms involved in the blue light inactivation of <jats:italic>P. aeruginosa<\/jats:italic>.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202300384-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202300384","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:56:03Z","timestamp":1701132963000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A multimodal nonlinear optical microscopy study of the responses of <i>Pseudomonas aeruginosa<\/i> to blue light and antibiotic treatment"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7735-5614","authenticated-orcid":false,"given":"Chi","family":"Zhang","sequence":"first","affiliation":[{"name":"Beckman Institute for Advanced Science and Technology University of Illinois Urbana\u2010Champaign  Urbana Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farzana R.","family":"Zaki","sequence":"additional","affiliation":[{"name":"Beckman Institute for Advanced Science and Technology University of Illinois Urbana\u2010Champaign  Urbana Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1007-4049","authenticated-orcid":false,"given":"Jungeun","family":"Won","sequence":"additional","affiliation":[{"name":"Beckman Institute for Advanced Science and Technology University of Illinois Urbana\u2010Champaign  Urbana Illinois USA"},{"name":"Department of Bioengineering University of Illinois Urbana\u2010Champaign  Urbana Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9386-5630","authenticated-orcid":false,"given":"Stephen A.","family":"Boppart","sequence":"additional","affiliation":[{"name":"Beckman Institute for Advanced Science and Technology University of Illinois Urbana\u2010Champaign  Urbana Illinois USA"},{"name":"Department of Bioengineering University of Illinois Urbana\u2010Champaign  Urbana Illinois USA"},{"name":"Department of Electrical and Computer Engineering University of Illinois Urbana\u2010Champaign  Urbana Illinois USA"},{"name":"Cancer Center at Illinois University of Illinois Urbana\u2010Champaign  Urbana Illinois USA"},{"name":"Carle Illinois College of Medicine University of Illinois Urbana\u2010Champaign  Urbana Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/5.2.279"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2009.04.020"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00065"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2018.11.013"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504266102"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.4172\/1989-8436.100083"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJB07.613"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3009650"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-016-0028-x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9LC00502A"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18902-x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202001452"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b03382"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20948"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.2174\/1574891X113089990012"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2016.1250995"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2006.24.680"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01652-12"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-019-0470-1"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2020.105024"},{"key":"e_1_2_9_22_1","doi-asserted-by":"crossref","unstructured":"S. L.White US Patent 8 021 405.2011.","DOI":"10.1088\/1475-7516\/2011\/05\/021"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2012.3365"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2008.2413"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201900030"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201903117"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c03366"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2013.08.039"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12544"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079111"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10010009"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M609238200"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.15698\/mic2018.10.649"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-56913-x"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-016-0001-8"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-85795-1"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.14152"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1213180"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphy.2021.642302"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat4720"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583519000155"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.03317"},{"key":"e_1_2_9_43_1","doi-asserted-by":"crossref","DOI":"10.1128\/mSphere.00401-21","volume":"6","author":"Kang D.","year":"2021","journal-title":"mSphere"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2004.10.002"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.05.009"},{"key":"e_1_2_9_46_1","first-page":"23","volume":"13","author":"Das T.","year":"2016","journal-title":"Microbial Biofilms\u2010Importance Appl."},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio764"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics9010022"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2036282100"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01479-12"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202300384","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202300384","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,25]],"date-time":"2024-03-25T02:30:36Z","timestamp":1711333836000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.202300384"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,15]]},"references-count":49,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1002\/jbio.202300384"],"URL":"https:\/\/doi.org\/10.1002\/jbio.202300384","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12,15]]},"assertion":[{"value":"2023-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202300384"}}