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Despite having precursor conditions like peripheral arterial disease (PAD), they are often only diagnosed after the onset of stroke or heart attack. Low\u2010cost, portable, noninvasive, point\u2010of\u2010care (POC), label\u2010free assessment of deep vascular function benefits PAD diagnosis, especially in resource poor settings of the world. Doppler ultrasound\u2010based blood flow measurements can diagnose PAD, albeit with limited sensitivity and specificity. To overcome this, here, we propose the first\u2010of\u2010its\u2010kind dual\u2010modality photoacoustic\u2010and\u2010ultrasound (PAUS) imaging system that integrates a multiwavelength pulsed laser diode (PLD) with a compact ultrasound data acquisition unit. The mesoscopic imaging depth of the portable PLD\u2010PAUS system was validated using tissue phantoms, and its multispectral photoacoustic imaging capabilities were validated using an atherosclerosis\u2010mimicking phantom. Furthermore, we demonstrated high\u2010contrast volumetric in vivo photoacoustic imaging of rodent abdominal vasculature and quantified vessel reactivity due to hypercapnia stimulation. The multiparametric functional and molecular imaging capabilities of the PLD\u2010PAUS system holds promise for POC applications.<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/jbio202400058-gra-0001.png\" xlink:title=\"image\"\/><\/jats:p>","DOI":"10.1002\/jbio.202400058","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T11:35:38Z","timestamp":1714649738000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Multiwavelength laser diode based portable photoacoustic and ultrasound imaging system for point of care applications"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3408-5176","authenticated-orcid":false,"given":"Mahaan","family":"Mitra","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering The Pennsylvania State University  University Park Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abigail","family":"Haworth","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering The Pennsylvania State University  University Park Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prameth","family":"Gaddale","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering The Pennsylvania State University  University Park Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faisal","family":"Badran","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering The Pennsylvania State University  University Park Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuzhath","family":"Lagno","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering The Pennsylvania State University  University Park Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colette","family":"Pameijer","sequence":"additional","affiliation":[{"name":"Divison of Vascular Surgery, Department of Surgery The Pennsylvania State University, College of Medicine  Hershey Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faisal","family":"Aziz","sequence":"additional","affiliation":[{"name":"Divison of Vascular Surgery, Department of Surgery The Pennsylvania State University, College of Medicine  Hershey Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sri\u2010Rajasekhar","family":"Kothapalli","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering The Pennsylvania State University  University Park Pennsylvania USA"},{"name":"Center for Neural Engineering The Pennsylvania State University  University Park Pennsylvania USA"},{"name":"Penn State Cancer Institute The Pennsylvania State University, College of Medicine  Hershey Pennsylvania USA"},{"name":"Graduate Program in Acoustics The Pennsylvania State University  University Park Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,2]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Stroke: Pathophysiology, Diagnosis, and Management","author":"Mendelow A. 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