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The architecture and biomechanical properties of the limbus have previously been demonstrated to change due to aging and disease. This study aims to non\u2010destructively and simultaneously quantify these limbal niche properties, along with their age\u2010related changes. A lab\u2010built vibrational optical coherence elastography (OCE) device consisting of a phase\u2010sensitive optical coherence tomography (OCT) with a vibrational stimulator has been used to collect structural images and the depth\u2010resolved elasticity of human corneoscleral tissues (aged 4\u201396\u2009years old). The limbal palisades of Vogt (POV) were delineated well in OCT images which were validated by histology. The POVs have been spatially mapped with simultaneous elasticity measurements in cross\u2010sections, showing tissue stiffness distributions across the undulations. A significant influence of age on the dimensions of the POVs was explored. The elastic modulus within the limbal niches for the \u226565\u2010year\u2010old group was significantly higher than that of the &lt;65\u2010year\u2010old group.<\/jats:p>","DOI":"10.1002\/jbio.202400172","type":"journal-article","created":{"date-parts":[[2024,9,28]],"date-time":"2024-09-28T06:11:27Z","timestamp":1727503887000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterizing Biomechanics of Limbal Niche Using Vibrational Optical Coherence Elastography"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-4221-7362","authenticated-orcid":false,"given":"Ryan","family":"Dimmock","sequence":"first","affiliation":[{"name":"School of Pharmacy and Bioengineering Keele University  Stoke\u2010on\u2010Trent UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yilong","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Science and Engineering University of Dundee  Dundee UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gibran F.","family":"Butt","sequence":"additional","affiliation":[{"name":"Ophthalmology, Institute of Inflammation and Ageing University of Birmingham  Birmingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saaeha","family":"Rauz","sequence":"additional","affiliation":[{"name":"Ophthalmology, Institute of Inflammation and Ageing University of Birmingham  Birmingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihong","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Science and Engineering University of Dundee  Dundee UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Bioengineering Keele University  Stoke\u2010on\u2010Trent UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,9,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2006.102640"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-18090-2"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.18-25740"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-26677-9"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtos.2016.04.002"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0094283"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0580"},{"key":"e_1_2_8_9_1","first-page":"155","article-title":"Limbal Palisades of Vogt","volume":"80","author":"Goldberg M. 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