{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T15:01:25Z","timestamp":1781708485137,"version":"3.54.5"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T00:00:00Z","timestamp":1733961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Androgenetic alopecia (AGA) causes balding in approximately 50% of adults. One primary cause of AGA is synthesis of dihydrotestosterone from testosterone by 5\u2010\u03b1 reductase. Systemic pharmaceutical interventions have potentially serious side effects, necessitating development of localized interventions. One such approach is administration of red light via low level light therapy (LLLT), which has promising clinical data. However, the LLLT mechanism of action remains unclear. We investigated the ability of LLLT to stimulate nitric oxide (NO) and the role of NO in inhibition of DHT synthesis. Our results show that red and red\u2010orange light induce NO release in a cell\u2010free platform. In A549 and HEK293T cells, we demonstrate 620 and 660\u2009nm LED\u2010emitted light stimulates the production of NO, reactive oxygen species (ROS), and decreases DHT synthesis. These results provide a plausible mechanism of action for LLLT employing LED\u2010emitted red and red\u2010orange wavelengths of light to treat AGA.<\/jats:p>","DOI":"10.1002\/jbio.202400388","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T00:20:12Z","timestamp":1734049212000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dual Wavelength <scp>LEDs<\/scp> Induce Reactive Oxygen Species and Nitric Oxide That Inhibit the Production of Dihydrotestosterone by 5\u2010\u03b1 Reductase"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6768-3449","authenticated-orcid":false,"given":"Jacob","family":"Kocher","sequence":"first","affiliation":[{"name":"Revian  Morrisville North Carolina USA"},{"name":"KNOW Bio LLC  Morrisville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Jandick","sequence":"additional","affiliation":[{"name":"Revian  Morrisville North Carolina USA"},{"name":"KNOW Bio LLC  Morrisville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derry","family":"Spragion","sequence":"additional","affiliation":[{"name":"Revian  Morrisville North Carolina USA"},{"name":"KNOW Bio LLC  Morrisville North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"P. 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