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Ultraviolet\u2010A (UVA) crosslinking has emerged as the primary treatment to slow down collagen degradation. This treatment is limited in both penetration depth and spatial precision, potentially leading to unwanted side effects. This study compares the changes in biomechanical properties of corneas crosslinked with UVA irradiation and a near\u2010infrared femtosecond laser, using Brillouin microscopy. The biomechanical properties of the crosslinked regions were mapped in terms of Brillouin frequency shift in three dimensions. UVA crosslinking showed an average increase in Brillouin frequency shift of ~100\u2009MHz. We demonstrate targeted spatial and axial corneal femtosecond crosslinking, with similar Brillouin frequency shift values to UVA in crosslinked regions.<\/jats:p>","DOI":"10.1002\/jbio.202400401","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T02:10:01Z","timestamp":1739758201000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Assessing <scp>UVA<\/scp> and Laser\u2010Induced Crosslinking via Brillouin Microscopy"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1246-9936","authenticated-orcid":false,"given":"Christian A.","family":"Iriarte\u2010Valdez","sequence":"first","affiliation":[{"name":"Institute of Quantum Optics Leibniz University Hannover  Hannover Germany"},{"name":"Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Wenzel","sequence":"additional","affiliation":[{"name":"Institute of Quantum Optics Leibniz University Hannover  Hannover Germany"},{"name":"Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Baron","sequence":"additional","affiliation":[{"name":"Institute of Quantum Optics Leibniz University Hannover  Hannover Germany"},{"name":"Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra Y.","family":"Claus","sequence":"additional","affiliation":[{"name":"Institute of Quantum Optics Leibniz University Hannover  Hannover Germany"},{"name":"Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Kalies","sequence":"additional","affiliation":[{"name":"Institute of Quantum Optics Leibniz University Hannover  Hannover Germany"},{"name":"Lower Saxony Center for Biomedical Engineering Implant Research and Development (NIFE)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"Sperlich","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Rostock University Medical Center  Rostock Germany"},{"name":"Department of Life, Light &amp; 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