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Serum samples from control and alcohol use disorder (AUD) were analyzed, and their spectra were compared. Multivariate analysis techniques, including Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA), were employed to differentiate the groups. A machine learning model, Grid Search\u2010Support Vector Machine Discriminant Analysis (GS\u2010SVMDA), was developed to classify samples with high accuracy. Significant differences in the absorbance values of specific functional groups, particularly those associated with phospholipids, amides, and fatty acids revealed. The AUD exhibited lower levels of these biomolecules. The models achieved perfect classification, demonstrating the potential ofFTIR spectroscopy as a non\u2010invasive tool for diagnosing AUD. Findings contribute to a better understanding of the biochemical mechanisms underlying alcohol addiction and may aid in the development of novel diagnostic and therapeutic strategies.<\/jats:p>","DOI":"10.1002\/jbio.202400550","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T06:40:14Z","timestamp":1741761614000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Investigating the Impact of Long\u2010Term Alcohol Consumption on Serum Chemical Changes: Fourier Transform Infrared Spectroscopy for Human Blood Serum"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4136-4447","authenticated-orcid":false,"given":"Zozan","family":"Guleken","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Gaziantep Islam, Science and Technology University  Gaziantep Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3301-3708","authenticated-orcid":false,"given":"Devrim","family":"Sar\u0131bal","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Cerrahpasa Faculty of Medicine Istanbul University\u2010Cerrahpa\u015fa  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2546-8254","authenticated-orcid":false,"given":"Hasan","family":"M\u0131rsal","sequence":"additional","affiliation":[{"name":"Department of Mental Health and Diseases Bal\u0131kl\u0131 Rum Hospital  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5657-5354","authenticated-orcid":false,"given":"Jozef","family":"Cebulski","sequence":"additional","affiliation":[{"name":"Institute of Physics University of Rzeszow  Rzesz\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeynep","family":"Ceylan","sequence":"additional","affiliation":[{"name":"Faculty of Engineering, Department of Industrial Engineering Samsun University  Samsun Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0168-1701","authenticated-orcid":false,"given":"Joanna","family":"Depciuch","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Medical University of Lublin  Lublin Poland"},{"name":"Institute of Nuclear Physics, PAS  Krak\u00f3w Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21591"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(06)67924\u20105"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.j2353"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.2217\/whe.14.62"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21903"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.14309\/ajg.0000000000000340"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010pathmechdis\u2010031521\u2010030435"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph17072610"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2017.01.017"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/10911359.2011.566802"},{"key":"e_1_2_11_12_1","unstructured":"WHO \u201cHarmful Use of Alcohol \u201dinNMH Fact Sheet accessed May 28 2024 http:\/\/www.who.int\/nmh\/publications\/fact_sheet_alcohol_en.pdf."},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs1020345"},{"key":"e_1_2_11_14_1","first-page":"7","article-title":"Alcoholic Liver Disease: Pathogenesis and Current Management","volume":"38","author":"Osna N. 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