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Accurate and rapid detection of psoriasis is crucial for diagnosis and treatment. Herein, we utilized multiphoton microscopy (MPM) to rapidly image psoriasis in imiquimod\u2010induced mouse and human psoriatic samples. Our results showed that MPM accurately monitored the thickening of stratum corneum and epidermis in psoriatic tissues, similar to traditional H&amp;E staining. Notably, we observed an increased alignment and number of collagen fibers in both mouse models and human psoriatic samples. Furthermore, a decrease in the proportionate area, length, width, and cross\u2010linking gap of collagen fibers, as well as an increase in cross\u2010linking density was also obtained in mouse psoriatic models. These findings suggest that collagen can be a biomarker for evaluating the progress of psoriasis. 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