{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T15:46:17Z","timestamp":1782488777930,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Brucellosis is a notable zoonotic disease caused by Brucella that is often overlooked. Diagnosis involves both clinical symptoms and serological examinations, which are accurate but time\u2010consuming. Therefore, a simple and accurate method is needed. This study aims to assess the potential for diagnosing human brucellosis using serum fluorescence spectra in conjunction with principal component analysis\u2013linear discriminant analysis (PCA\u2010LDA), linear support vector machine (linear SVM), medium radial basis function support vector machine (RBF SVM), K\u2010nearest neighbors (KNN), and decision tree (DT). The study of serum fluorescence spectra in brucellosis\u2010infected compared to healthy revealed that patients with brucellosis had reduced peaks at 452, 624, and 688\u2009nm and elevated peaks at 495 and 643\u2009nm. SVM (linear\/RBF) provides more accurate classification results than other algorithms. The method achieved an overall diagnostic accuracy of 89.0%. In conclusion, the serum fluorescence spectrum paired with the SVM (linear\/RBF) algorithm is highly promising for human brucellosis detection.<\/jats:p>","DOI":"10.1002\/jbio.202500100","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T22:13:27Z","timestamp":1751580807000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Rapid and Noninvasive Detection of Brucellosis in Human Based on Serum Fluorescence Spectrum Combined With Machine Learning Algorithms"],"prefix":"10.1002","volume":"18","author":[{"given":"Ziyi","family":"Fang","sequence":"first","affiliation":[{"name":"College of Life Sciences and Technology, Xinjiang University  Urumqi China"},{"name":"State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Wang","sequence":"additional","affiliation":[{"name":"The Sixth Hospital for the People in the Xinjiang Uygur Autonomous Region  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwei","family":"Gong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangxiang","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Electrical Engineering and Automation, Tianjin University of Technology  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wubulitalifu","family":"Dawuti","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics School of Public Health, Peking University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengke","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Technology, Xinjiang University  Urumqi China"},{"name":"State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[{"name":"The First Clinical College of Xinjiang Medical University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1770-574X","authenticated-orcid":false,"given":"Guodong","family":"L\u00fc","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Technology, Xinjiang University  Urumqi China"},{"name":"State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia Clinical Medical Research Institute, The First Affiliated Hospital of Xinjiang Medical University  Urumqi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"issue":"1","key":"e_1_2_9_2_1","article-title":"Pathophysiology, Current Therapeutic Options, Vaccine Candidates, and Drug Targets for Human Brucellosis","volume":"17","author":"Pritam M.","year":"2024","journal-title":"Current Molecular Pharmacology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2909.230052"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70382-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-017-5016-y"},{"key":"e_1_2_9_6_1","volume-title":"The Control of Neglected Zoonotic Diseases: From Advocacy to Action: Report of the Fourth International Meeting Held at WHO Headquarters, Geneva, Switzerland, 19\u201320 November 2014","author":"World Health","year":"2015"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2023.2290839"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-021-05973-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.2196\/42820"},{"issue":"6","key":"e_1_2_9_10_1","first-page":"114","article-title":"Epidemiological Characteristics of Human Brucellosis \u2010 China, 2016\u20102019","volume":"3","author":"Tao Z.","year":"2021","journal-title":"China CDC Weekly"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1136674"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010468"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2019.108775"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10121623"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00073-19"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2018.05.012"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-7-99"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2019.101598"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.10.003009"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijsu.2018.05.029"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiomed.2024.109251"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202200354"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2021.102276"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202300357"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2014.03.009"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox14010083"},{"issue":"1","key":"e_1_2_9_27_1","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s12094-024-03564-8","article-title":"Enhancing the Diagnostic Accuracy of Colorectal Cancer Through the Integration of Serum Tumor Markers and Hematological Indicators With Machine Learning Algorithms","volume":"27","author":"Xu R.","year":"2025","journal-title":"Clinical & Translational Oncology: Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/cts.13619"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2024.104426"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/b922045c"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2023.103544"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/s21144623"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2022.102811"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2023.103567"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-30241-7"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1562\/0031-8655(2003)0780197NFSOBP2.0.CO2"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2024.116370"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.17.9.098001"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202300376"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2023.122354"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10916-005-5187-4"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2022.103102"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acra.2024.02.009"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.202500100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T23:23:26Z","timestamp":1759965806000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.202500100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,3]]},"references-count":42,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1002\/jbio.202500100"],"URL":"https:\/\/doi.org\/10.1002\/jbio.202500100","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,3]]},"assertion":[{"value":"2025-02-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202500100"}}