{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:11:29Z","timestamp":1763338289587,"version":"3.45.0"},"reference-count":13,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Photoacoustic computed tomography (PACT) synergizes optical absorption contrast with ultrasonic resolution for noninvasive biomedical imaging yet faces limitations in signal\u2010to\u2010noise ratio (SNR), resolution, and contrast. This study introduces a coherent\u2010excitation PACT system integrating interferometric optical excitation and Frequency\u2010Compensated Filtered Back Projection (FC\u2010FBP) reconstruction. The proposed method utilizes phase\u2010locked dual\u2010pulse interferometric excitation to amplify photoacoustic emissions; for the isolated chicken heart, the resolution is improved by 7.9% compared to the single\u2010pulse protocol. The FC\u2010FBP algorithm compensates for frequency\u2010dependent acoustic attenuation via depth\u2010adaptive Gaussian filtering, enhancing the projected signal in the target area while suppressing speckle artifacts. Through experimental validation, we confirm that the coherent\u2010excitation scheme enables simultaneous optimization of optical fluence distribution and acoustic coherence; hence, it can be used to resolve previously indistinguishable hemoglobin oxygenation gradients in murine tumor models. This advancement establishes a high\u2010sensitivity PACT framework, showing potential for real\u2010time intraoperative imaging and dynamic metabolic monitoring in clinical applications.<\/jats:p>","DOI":"10.1002\/jbio.202500175","type":"journal-article","created":{"date-parts":[[2025,8,14]],"date-time":"2025-08-14T01:10:31Z","timestamp":1755133831000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Coherent\u2010Excitation\n                    <scp>PACT<\/scp>\n                    With Frequency\u2010Compensated Reconstruction for High\u2010Contrast Deep\u2010Tissue Imaging"],"prefix":"10.1002","volume":"18","author":[{"given":"Ruijie","family":"Hou","sequence":"first","affiliation":[{"name":"School of Electronic and Optical Engineering Nanjing University of Science and Technology  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Electronic and Optical Engineering Nanjing University of Science and Technology  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Artificial Intelligence Luoyang Institute of Science and Technology  Luoyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Hua","sequence":"additional","affiliation":[{"name":"School of Electronic and Optical Engineering Nanjing University of Science and Technology  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Electronic and Optical Engineering Nanjing University of Science and Technology  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuefeng","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Electronic and Optical Engineering Nanjing University of Science and Technology  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2793-2137","authenticated-orcid":false,"given":"Muhammad Qasim","family":"Mehmood","sequence":"additional","affiliation":[{"name":"MicroNano Lab Information Technology University of the Punjab (ITU)  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7051-802X","authenticated-orcid":false,"given":"Jichuan","family":"Xiong","sequence":"additional","affiliation":[{"name":"School of Electronic and Optical Engineering Nanjing University of Science and Technology  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,13]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_10_2_1","DOI":"10.1126\/science.1216210"},{"doi-asserted-by":"publisher","key":"e_1_2_10_3_1","DOI":"10.1118\/1.4824317"},{"doi-asserted-by":"publisher","key":"e_1_2_10_4_1","DOI":"10.1364\/JOSAA.33.002333"},{"doi-asserted-by":"publisher","key":"e_1_2_10_5_1","DOI":"10.1364\/BOE.6.004118"},{"doi-asserted-by":"publisher","key":"e_1_2_10_6_1","DOI":"10.3390\/app8091570"},{"doi-asserted-by":"publisher","key":"e_1_2_10_7_1","DOI":"10.1117\/12.709865"},{"doi-asserted-by":"publisher","key":"e_1_2_10_8_1","DOI":"10.1118\/1.2426406"},{"doi-asserted-by":"publisher","key":"e_1_2_10_9_1","DOI":"10.1080\/17415977.2018.1518444"},{"doi-asserted-by":"publisher","key":"e_1_2_10_10_1","DOI":"10.1117\/1.JBO.22.4.041003"},{"doi-asserted-by":"publisher","key":"e_1_2_10_11_1","DOI":"10.1364\/OE.401708"},{"doi-asserted-by":"publisher","key":"e_1_2_10_12_1","DOI":"10.1088\/1361-6463\/abc37d"},{"doi-asserted-by":"publisher","key":"e_1_2_10_13_1","DOI":"10.1038\/s41467-019-11024-z"},{"doi-asserted-by":"publisher","key":"e_1_2_10_14_1","DOI":"10.2352\/ISSN.2470-1173.2017.15.DPMI-079"}],"container-title":["Journal 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