{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T09:48:41Z","timestamp":1769680121621,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,24]],"date-time":"2025-08-24T00:00:00Z","timestamp":1755993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,8,24]],"date-time":"2025-08-24T00:00:00Z","timestamp":1755993600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100023984","name":"Raja Ramanna Centre for Advanced Technology, Department of Atomic Energy, Government of India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100023984","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017632","name":"Homi Bhabha National Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017632","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Optogenetics offers a promising avenue for controlled insulin secretion in diabetes management using genetically modified cells. However, the influence of irradiation parameters on the co\u2010secreted hormone amylin remains largely unexplored. This study examined how blue light power densities (50, 100, and 200\u2009mW\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ) under continuous and intermittent exposure affect hormonal secretion in channelrhodopsin\u20102 expressing mouse \u03b2 cells. Results showed that 10\u2009s of continuous exposure at 50\u2009mW\/cm\n                    <jats:sup>2<\/jats:sup>\n                    increased insulin and amylin secretion by ~25% and 40%, respectively. Introducing intermittent dark intervals further enhanced secretion by ~35% for insulin and 70% for amylin at 50 and 100\u2009mW\/cm\n                    <jats:sup>2<\/jats:sup>\n                    respectively, correlating with intracellular calcium changes. Notably, light modulated the amylin\u2010to\u2010insulin molar ratio, peaking with a 34% increase during 5\u2009s \u2018on\u2019\u201010s \u2018off\u2019 exposure at 100\u2009mW\/cm\n                    <jats:sup>2<\/jats:sup>\n                    . This study provides the first evidence that light irradiation parameters can differentially influence hormonal secretion from engineered cells, offering a potential for tailored cell\u2010based diabetes therapies.\n                  <\/jats:p>","DOI":"10.1002\/jbio.202500232","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T05:04:54Z","timestamp":1756098294000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Light\u2010Mediated Insulin and Amylin Secretion: Influence of Irradiation Parameters in Optogenetically Modified Mouse\u2010Pancreatic \u03b2 Cells"],"prefix":"10.1002","volume":"19","author":[{"given":"Nidhi","family":"Maharwal","sequence":"first","affiliation":[{"name":"Laser Biomedical Applications Division Raja Ramanna Centre for Advanced Technology  Indore India"},{"name":"Homi Bhabha National Institute BARC Training School Complex  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3324-1316","authenticated-orcid":false,"given":"Rashmi","family":"Shrivastava","sequence":"additional","affiliation":[{"name":"Laser Biomedical Applications Division Raja Ramanna Centre for Advanced Technology  Indore India"},{"name":"Homi Bhabha National Institute BARC Training School Complex  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4633-1134","authenticated-orcid":false,"given":"Khageswar","family":"Sahu","sequence":"additional","affiliation":[{"name":"Laser Biomedical Applications Division Raja Ramanna Centre for Advanced Technology  Indore India"},{"name":"Homi Bhabha National Institute BARC Training School Complex  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shovan Kumar","family":"Majumder","sequence":"additional","affiliation":[{"name":"Laser Biomedical Applications Division Raja Ramanna Centre for Advanced Technology  Indore India"},{"name":"Homi Bhabha National Institute BARC Training School Complex  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20102467"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.4239\/wjd.v7.i9.189"},{"key":"e_1_2_9_4_1","first-page":"108","article-title":"Recent Advance in Diabetes Therapy: Pancreatic Beta Cell Regeneration Approaches","volume":"6","author":"Chala T. 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