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Hyperspectral imaging (HSI) is emerging as a promising label\u2010free technique for rapid biochemical tissue assessment. Here, we present HyperProbe1.1 (HP1.1), an HSI system designed for noninvasive analysis of fresh brain tumor biopsies. In this proof\u2010of\u2010concept study, we applied the HP1.1 system to freshly excised meningioma specimens\u2014the most common primary intracranial tumors. The platform enabled rapid, label\u2010free mapping of metabolic activity and vascular heterogeneity, while spectral unmixing further allowed the quantification of endogenous biomarkers such as cytochrome c oxidase (CCO), hemoglobin derivatives, and lipids, revealing molecular patterns consistent with histopathological tumor grading according to the 2021 WHO classification. These results highlight the feasibility of HSI for rapid biochemical tissue assessment and its potential integration into intraoperative decision\u2010making.<\/jats:p>","DOI":"10.1002\/jbio.202500374","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T01:20:24Z","timestamp":1765848024000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Label\u2010Free Hyperspectral Imaging Device for Ex\u00a0Vivo Characterization and Grading of Meningioma Tissues"],"prefix":"10.1002","volume":"19","author":[{"given":"Pietro","family":"Ricci","sequence":"first","affiliation":[{"name":"Department of Physics and Astronomy University of Florence Sesto Fiorentino Italy"},{"name":"European Laboratory for Non\u2010Linear Spectroscopy Sesto Fiorentino 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