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Dynamic optical coherence tomography (D\u2010OCT), an imaging method that enables noninvasive assessment of microvasculature, in conjunction with automated quantitative analysis, shows promise as an objective and robust method for monitoring AKs. Critical to quantitative longitudinal assessments is a dataset in which good\u2010quality images are available at all time points. Yet, to date, few resources exist to train clinical researchers on how to ensure robust D\u2010OCT data collection. In this study, we identify hallmarks of poor image quality to guide data curation and avoid biasing study results. We highlight strategies for imaging lesions with significant hyperkeratosis. Finally, we discuss characteristic features present in D\u2010OCT images of AK that may complicate quantitative analysis. We hope that this work will facilitate the use of D\u2010OCT in the objective longitudinal assessment of AK.<\/jats:p>","DOI":"10.1002\/jbio.70240","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T11:12:34Z","timestamp":1772968354000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Longitudinal Assessment of Vascularity in Actinic Keratosis Using Dynamic Optical Coherence Tomography: Practical Considerations and Theoretical Insights"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6334-0625","authenticated-orcid":false,"given":"Gavrielle R.","family":"Untracht","sequence":"first","affiliation":[{"name":"Department of Dermatology Copenhagen University Hospital \u2010 Bispebjerg and Frederiksberg  Copenhagen Denmark"},{"name":"Department of Health Technology Technical University of Denmark  Kongens Lyngby Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3903-2651","authenticated-orcid":false,"given":"Gabriella","family":"Fredman","sequence":"additional","affiliation":[{"name":"Department of Dermatology Copenhagen University Hospital \u2010 Bispebjerg and Frederiksberg  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3220-8303","authenticated-orcid":false,"given":"John R.","family":"Zibert","sequence":"additional","affiliation":[{"name":"Coegin Pharma AB  Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1250-2035","authenticated-orcid":false,"given":"Merete","family":"Haedersdal","sequence":"additional","affiliation":[{"name":"Department of Dermatology Copenhagen University Hospital \u2010 Bispebjerg and Frederiksberg  Copenhagen Denmark"},{"name":"Department of Clinical Medicine University of Copenhagen  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9708-6717","authenticated-orcid":false,"given":"Vinzent Kevin","family":"Ortner","sequence":"additional","affiliation":[{"name":"Department of Dermatology Copenhagen University Hospital \u2010 Bispebjerg and Frederiksberg  Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,3]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.19897"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bjd\/ljad371"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abd.2019.10.004"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12955-015-0295-4"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.15107"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.2340\/actadv.v103.6229"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.36849\/JDD.6704"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.5826\/dpc.1403S1a146S"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000444706"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0261052"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-26871-y"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.2340\/actadv.v104.42190"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.537727"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000502458"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3928\/23258160-20230206-01"},{"key":"e_1_2_12_17_1","first-page":"31","article-title":"An Open\u2010Source Image Analysis Toolbox for Quantitative Retinal Optical Coherence Tomography Angiography","volume":"14","author":"Amirmoezzi Y.","year":"2021","journal-title":"Journal of Biomedical Physics & Engineering"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40942-022-00426-9"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-024-03022-z"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.15068"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2022-061012"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/IAE.0000000000000765"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.18502\/jovr.v16i2.9091"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/ceo.12683"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.8.001056"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.7.000911"},{"key":"e_1_2_12_27_1","unstructured":"G. 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