{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T01:46:01Z","timestamp":1774921561063,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001823","name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007397","name":"Univerzita Karlova v Praze","doi-asserted-by":"publisher","award":["SVV 2026 260 773"],"award-info":[{"award-number":["SVV 2026 260 773"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>With their noninvasive capabilities for molecular\u2010level analysis of biological tissues and cells, Raman spectroscopy and microscopy have become important tools in medicine and medical diagnostics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this work, we describe a reliable and repeatable technique for Raman spectroscopic profiling of human neutrophils in peripheral blood smears. 1200 single\u2010cell spectra from 12 healthy donors were obtained using Raman microscopy, and the results showed consistent spectral features of key biomolecules such as proteins, nucleic acids, carotenoids, and cytochrome c.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We used t\u2010distributed stochastic neighbor embedding and principal component analysis to assess interindividual variability; both techniques showed minimal spectral divergence between donors and high reproducibility. This study lays the methodological groundwork for upcoming Raman microscopy applications enabling non\u2010invasive assessment of immune cell states in health and disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our results demonstrate the potential of Raman microscopy in clinical diagnostics, specifically for tracking the metabolic states of cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbio.70251","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T16:22:02Z","timestamp":1773159722000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Raman Spectral Signatures of Human Neutrophils: A Single\u2010Cell Approach"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9358-5049","authenticated-orcid":false,"given":"Lenka","family":"Va\u0148kov\u00e1","sequence":"first","affiliation":[{"name":"Faculty of Medicine in Pilsen Charles University in Prague  Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7853-195X","authenticated-orcid":false,"given":"Ji\u0159\u00ed","family":"Bufka","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Pilsen Charles University in Prague  Pilsen Czech Republic"},{"name":"Motol University Hospital  Prague Czech Republic"},{"name":"Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavla","family":"\u0160igutov\u00e1","sequence":"additional","affiliation":[{"name":"University Hospital in Pilsen  Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Holubov\u00e1","sequence":"additional","affiliation":[{"name":"University Hospital in Pilsen  Pilsen Czech Republic"},{"name":"Biomedical Center, Faculty of Medicine in Pilsen Charles University  Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u011bra","family":"K\u0159\u00ed\u017ekov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Medicine in Pilsen Charles University in Prague  Pilsen Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,10]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511535468"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpclett.2c00653"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18376-x"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.1c08370"},{"key":"e_1_2_12_6_1","volume-title":"Density\u2010Functional Theory of Atoms and Molecules","author":"Parr R. 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