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We expanded the application of our optical measurement system, the Transilluminated Quantum Dot (TIQD) imager, paired with a low\u2010cost biochip for minimally invasive biomolecule detection. Streptavidin functionalized quantum dots (Sav\u2010QDs) were conjugated with biotinylated antibodies (Ab) against stress biomarkers heat shock protein (Hsp). The presence of biomarkers in biological samples was indicated by the fluorescence masking of Sav\u2010QDs, based on their binding to the Abs conjugated to Sav\u2010QDs, resulting in reduced fluorescence. The decrease in fluorescence directly correlates with biomarker concentration. We have successfully detected Hsp90 and Hsp70 spiked in artificial blood serum. The limit of detection (LoD) was around 50\u2009nM for Hsp90 and 96\u2009nM for Hsp70. Our portable sensor addresses the need for reliable point\u2010of\u2010care (PoC) stress diagnosis by enabling robust, multiple biomarkers detection in diverse biological samples.<\/jats:p>","DOI":"10.1002\/jbio.70268","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T03:36:21Z","timestamp":1776656181000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Functionalized Quantum Dot\u2010Based Portable Sensor for Dual Detection of Stress Biomarkers in Blood Serum and Saliva"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2609-1564","authenticated-orcid":false,"given":"Anusha","family":"Kishore","sequence":"first","affiliation":[{"name":"BMWZ (Centre of Biomolecular Drug Research) Gottfried Wilhelm Leibniz University Hannover  Hannover 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