{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:39:17Z","timestamp":1790746757842,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2025J01065"],"award-info":[{"award-number":["2025J01065"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2025J01854"],"award-info":[{"award-number":["2025J01854"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study sought to develop an objective grading system for myelofibrosis through characterization of collagen architecture using label\u2010free multiphoton microscopy, thereby enabling quantitative, standardized, and automated assessment. A multiphoton imaging dataset encompassing diverse myelofibrosis grades was constructed. Five collagen features were extracted utilizing the \u201cCT\u2010FIRE\u201d toolbox, and a support vector machine (SVM) classifier integrating these features was developed for four\u2010class categorization. The model demonstrated high accuracy across all myelofibrosis grades, with area under the curve (AUC) values of 0.994, 0.956, 0.940, and 1.000, and a macro\u2010average value of 0.973, thus achieving automated grading of myelofibrosis. This study quantified collagen content and fiber morphology in human bone marrow tissues, which facilitates objective myelofibrosis grading. Moreover, it lays a foundation for future automatic and rapid grading of myelofibrosis, which can subsequently be applied to the assessment of fibrosis in other organs.<\/jats:p>","DOI":"10.1002\/jbio.70269","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T04:50:58Z","timestamp":1776747058000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Second Harmonic Generation\u2010Based Collagen Analysis and Automated Grading of Myelofibrosis"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-4848-8810","authenticated-orcid":false,"given":"Xunbin","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Pathology Fuzhou University Affiliated Provincial Hospital  Fuzhou Fujian China"},{"name":"Shengli Clinical Medical College Fujian Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guodong","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Pathology Fuzhou University Affiliated Provincial Hospital  Fuzhou Fujian China"},{"name":"Shengli Clinical Medical College Fujian Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Fuzhou University Affiliated Provincial Hospital  Fuzhou Fujian China"},{"name":"Shengli Clinical Medical College Fujian Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology Fuzhou University Affiliated Provincial Hospital  Fuzhou Fujian China"},{"name":"Shengli Clinical Medical College Fujian Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingxin","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Arts and Sciences Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianhuang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, College of Photonic and Electronic Engineering Fujian Normal University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2024.03.006"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-03-643544"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41408-018-0054-y"},{"issue":"8","key":"e_1_2_13_5_1","first-page":"1128","article-title":"European Consensus on Grading Bone Marrow Fibrosis and Assessment of Cellularity","volume":"90","author":"Thiele J.","year":"2005","journal-title":"Haematologica"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12871"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13244-019-0758-y"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.18383\/j.tom.2016.00115"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40644-021-00405-7"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201200092"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.77532"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.23937"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13378"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7113-8_28"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.19.1.016007"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00198"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S195470"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.14846"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/pore.2022.1610217"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/00037028211063513"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.b.10031"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.02.015"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.70258"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/2632010X231162317"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep04636"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.modpat.2023.100155"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41232-026-00405-9"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dsp.2025.105863"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.APN.4.3.037001"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.22.2.026003"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.70269","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbio.70269","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.70269","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T04:37:02Z","timestamp":1777264622000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.70269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/jbio.70269"],"URL":"https:\/\/doi.org\/10.1002\/jbio.70269","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2025-12-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70269"}}