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The liver is one of the organs seriously affected by the disease as it lodges\n                      <jats:italic>Schistosoma<\/jats:italic>\n                      eggs. We show results by nonlinear imaging techniques, Second Harmonic Generation and Two\u2010Photon Excitation Fluorescence, to characterize mice tissues infected with\n                      <jats:italic>Schistosoma mansoni<\/jats:italic>\n                      in an experimental model. We followed the evolution of the disease in liver tissue sections through three stages: 30, 60, and 120\u2009days post\u2010infection\u2014the pre\u2010patent, acute, and early chronic phases, respectively. The presence of the\n                      <jats:italic>Schistosoma<\/jats:italic>\n                      eggs causes an increase in the collagen content and also changes in its architecture. The results allow us to clearly evaluate the disease progress helping to improve the understanding of liver fibrosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Second Harmonic Generation and Two\u2010Photon Excitation Fluorescence microscopy allows to characterize mice tissues infected with\n                      <jats:italic>Schistosoma mansoni<\/jats:italic>\n                      in an experimental model.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jbio.70295","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T05:18:02Z","timestamp":1780636682000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nonlinear Imaging Allows to Characterize Granulomas and Fibrosis in Mice Tissue From Experimental Schistosomiasis"],"prefix":"10.1002","volume":"19","author":[{"given":"Gladystone Rocha","family":"da Fonseca","sequence":"first","affiliation":[{"name":"Departamento de F\u00edsica, Instituto de Ci\u00eancias Exatas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rennan R. M.","family":"Lima","sequence":"additional","affiliation":[{"name":"Departamento de Biof\u00edsica e Radiobiologia Centro de Bioci\u00eancias, Universidade Federal de Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald Eduardo","family":"Soares","sequence":"additional","affiliation":[{"name":"Departamento de F\u00edsica, Instituto de Ci\u00eancias Exatas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e1rbara Regina Melo","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Departamento de F\u00edsica, Instituto de Ci\u00eancias Exatas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo E.","family":"Cabral Filho","sequence":"additional","affiliation":[{"name":"Departamento de Biof\u00edsica e Radiobiologia Centro de Bioci\u00eancias, Universidade Federal de Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Fontes","sequence":"additional","affiliation":[{"name":"Departamento de Biof\u00edsica e Radiobiologia Centro de Bioci\u00eancias, Universidade Federal de Pernambuco  Recife Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8551-5948","authenticated-orcid":false,"given":"Ana Maria","family":"de Paula","sequence":"additional","affiliation":[{"name":"Departamento de F\u00edsica, Instituto de Ci\u00eancias Exatas Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,4]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.36557\/2674\u20108169.2024v6n2p691\u2010711"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(22)00221\u20103"},{"key":"e_1_2_13_4_1","volume-title":"Guideline on Control and Elimination of Human Schistosomiasis","author":"WHO","year":"2022"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.1013354"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2017.06.025"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241210237"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2018.11.011"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00055\u201024"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aav3270"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201030582\u20103"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0174\u2010173X(84)80027\u20108"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/00034989958528"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155417718541"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10111585"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.21037\/qims\u201020\u2010394"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202002053RRR"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551\u2010022\u201001041\u20106"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering8020017"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.400871"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202200382"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10235521"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.906338"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.635513"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2018.02.005"},{"issue":"8","key":"e_1_2_13_26_1","first-page":"8599","article-title":"Chemiluminescent Detection of Glycocode Alterations in Hepatic Granulomatous Lesions of Experimental Schistosomiasis","volume":"10","author":"Nascimento G. 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