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Many ex\u00a0vivo studies rely on formalin\u2010embedded tissue specimens. Spectroscopic changes and classification of formalin\u2010fixed OSCC tissues were investigated. In an analysis of 47 specimens (\n                    <jats:italic>n\u2009=<\/jats:italic>\n                    \u200930 OSCC,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200917 physiological mucosa), RDS was employed at 180 distinct measurement loci after storage in saline, and again at 125 measurement loci after storage in formalin. For classification, Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) were utilized. In distinguishing OSCC from physiological mucosa, LDA revealed a higher sensitivity (97.6% for NaCl\u2010stored vs. 90.9% for formalin\u2010fixed tissues), specificity (93.0% vs. 78.6%), and overall classification accuracy (95.2% vs. 85.4%) for specimens stored in saline. While formalin fixation is valuable for tissue preservation in histopathology, Raman spectra of OSCC tissues were substantially altered, with implications for validation of innovative optical techniques such as Raman Spectroscopy.\n                  <\/jats:p>","DOI":"10.1002\/jbio.70326","type":"journal-article","created":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T10:43:26Z","timestamp":1785062606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Influence of Formaldehyde Fixation Media on the Raman Spectroscopic Analysis of Oral Squamous Cell Carcinoma"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7168-4428","authenticated-orcid":false,"given":"Levi","family":"Matthies","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasper F.","family":"Tegtmeyer","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Pessler","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Medhanie T.","family":"Gebrekidan","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Bergakademie Freiberg (TUBAF), Institute of Thermal\u2010, Environmental\u2010 and Resources Process Engineering (ITUN)  Freiberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina H.","family":"Besch","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Smeets","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"},{"name":"Department of Oral and Maxillofacial Surgery, Division of \u201cRegenerative Orofacial Medicine\u201d University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7816-4027","authenticated-orcid":false,"given":"Andreas S.","family":"Braeuer","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Bergakademie Freiberg (TUBAF), Institute of Thermal\u2010, Environmental\u2010 and Resources Process Engineering (ITUN)  Freiberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Gosau","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Knipfer","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf  Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,26]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMsr2210097"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33588"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/785929"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/326094"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.20226"},{"key":"e_1_2_13_8_1","unstructured":"\u201cL. 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