{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:10:55Z","timestamp":1789027855178,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Photodynamic therapy (PDT) is an established dermatologic therapeutic modality used to induce apoptosis within targeted tissue. Despite increasing clinical use, the genotoxic safety of blue light (BL) PDT has not yet been established. We examined whether combination BL (417\u2009\u00b1\u20095\u2009nm) and 5\u2010aminolevulinic acid (5\u2010ALA) PDT induces cyclobutane pyrimidine dimers (CPDs) and 6\u20134 photoproducts (6\u20134PPs) in human dermal fibroblasts. CRL\u20102617 and AG\u201013145 fibroblasts were treated with 0, 0.5, or 1\u2009mM 5\u2010ALA, then irradiated with BL at 10, 30, or 45\u2009J\/cm\n                    <jats:sup>2<\/jats:sup>\n                    . Unlike robust photolesion formation in the UVB\u2010irradiated positive controls, no BL PDT experimental condition produced CPD or 6\u20134PP signal exceeding the empirically validated assay lower limit of quantification (LLOQ; 1.5625\u2009ng\/mL). However, sub\u2010LLOQ DNA damage cannot be excluded. The minimum detectable effect was 2.42\u2009ng\/mL, ~1.55\u2010fold the LLOQ. Linear mixed\u2010effects sensitivity analysis confirmed assay responsiveness, demonstrating clear separation between UVB and BL PDT signals.\n                  <\/jats:p>","DOI":"10.1002\/jbio.70328","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T14:26:21Z","timestamp":1785939981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Blue Light Aminolevulinic Acid Photodynamic Therapy Did Not Induce Detectable\n                    <scp>CPD<\/scp>\n                    or 6\u2013\n                    <scp>4PP<\/scp>\n                    Photolesions in Human Dermal Fibroblasts"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3587-3998","authenticated-orcid":false,"given":"Julia","family":"Stolyar","sequence":"first","affiliation":[{"name":"Department of Dermatology State University of New York, Downstate Health Sciences University Brooklyn New York USA"},{"name":"Dermatology Services Veterans Affairs New York Harbor Healthcare System \u2013 Brooklyn Campus Brooklyn New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan","family":"Austin","sequence":"additional","affiliation":[{"name":"Department of Dermatology State University of New York, Downstate Health Sciences University Brooklyn New York USA"},{"name":"Dermatology Services Veterans Affairs New York Harbor Healthcare System \u2013 Brooklyn Campus Brooklyn New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0837-9604","authenticated-orcid":false,"given":"Olumide M.","family":"Arigbede","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics State University of New York, Downstate Health Sciences University Brooklyn New York USA"},{"name":"Office of the Senior Vice President for Research State University of New York, Downstate Health Sciences University Brooklyn New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7619-7650","authenticated-orcid":false,"given":"Jared","family":"Jagdeo","sequence":"additional","affiliation":[{"name":"Department of Dermatology State University of New York, Downstate Health Sciences University Brooklyn New York USA"},{"name":"Dermatology Services Veterans Affairs New York Harbor Healthcare System \u2013 Brooklyn Campus Brooklyn New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/DSS.0000000000000800"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2024.12.050"},{"key":"e_1_2_10_4_1","first-page":"44","article-title":"Clinical Efficacy of Self\u2010Applied Blue Light Therapy for Mild\u2010To\u2010Moderate Facial Acne","volume":"2","author":"Gold M. H.","year":"2009","journal-title":"Journal of Clinical and Aesthetic Dermatology"},{"key":"e_1_2_10_5_1","first-page":"46","article-title":"Treatment Satisfaction and Acceptability of 20% Aminolevulinic Acid Photodynamic Therapy for the Treatment of Actinic Keratoses of the Face, Scalp, and Upper Extremities","volume":"16","author":"Piacquadio D.","year":"2023","journal-title":"Journal of Clinical and Aesthetic Dermatology"},{"key":"e_1_2_10_6_1","volume-title":"Grand View Research","author":"Grand View R.","year":"2024"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2025.02.037"},{"key":"e_1_2_10_8_1","first-page":"36","article-title":"Light\u2010Emitting Diodes: A Brief Review and Clinical Experience","volume":"8","author":"Opel D. R.","year":"2015","journal-title":"Journal of Clinical and Aesthetic Dermatology"},{"key":"e_1_2_10_9_1","first-page":"32","article-title":"Safety and Efficacy of Aminolevulinic Acid 10% Topical Gel Versus Aminolevulinic Acid 20% Topical Solution Followed by Blue\u2010Light Photodynamic Therapy for the Treatment of Actinic Keratosis on the Face and Scalp: A Randomized, Double\u2010Blind Study","volume":"12","author":"Nestor M. S.","year":"2019","journal-title":"Journal of Clinical and Aesthetic Dermatology"},{"key":"e_1_2_10_10_1","first-page":"53","article-title":"Safety and Efficacy Comparison of Blue Versus Red Light Sources for Photodynamic Therapy Using Methyl Aminolevulinate in Photodamaged Skin","volume":"10","author":"Palm M. D.","year":"2011","journal-title":"Journal of Drugs in Dermatology"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/b201230h"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.800606"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1917196117"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202400510"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202200023"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.202300388"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2022.503562"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/phpp.12718"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology11121743"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.DSS.0000452624.01889.8a"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/lsm.22648"},{"key":"e_1_2_10_22_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2025"},{"key":"e_1_2_10_23_1","unstructured":"Food U.S. & Drug Administration \u201cBioanalytical Method Validation: Guidance for Industry \u201dCenter for Drug Evaluation and Research; Center for Veterinary Medicine (2018)."},{"key":"e_1_2_10_24_1","unstructured":"International Council for Harmonisation \u201cICH M10 Guideline: Bioanalytical Method Validation and Study Sample Analysis \u201dInternational Council for Harmonisation (2022)."},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/1948550617697177"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v067.i01"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v082.i13"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/1047322X.1990.10389587"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-022-00754-y"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2013.04.022"},{"key":"e_1_2_10_31_1","unstructured":"Cell Biolabs \u201cFAQ: Comet Assays\u201d."},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.01.029"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16054140"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.12368"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.70328","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbio.70328","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.70328","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T07:58:29Z","timestamp":1789027109000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.70328"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":33,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jbio.70328"],"URL":"https:\/\/doi.org\/10.1002\/jbio.70328","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2025-12-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70328"}}