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Its role in corneal biomechanics remains incompletely understood. Here, we investigated murine wild\u2010type (WT) and collagen XIV\u2010deficient (\n                    <jats:italic>Col14a1<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ) corneas using complementary optical elastography modalities: Brillouin microscopy and air\u2010coupled ultrasound optical coherence elastography (ACUS\u2010OCE). Brillouin microscopy generated high\u2010resolution maps of Brillouin frequency shift in WT and\n                    <jats:italic>Col14a1<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    corneas (\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u20094 per group), while ACUS\u2010OCE measured elastic wave speed in WT (\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u20098) and\n                    <jats:italic>Col14a1<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    (\n                    <jats:italic>N<\/jats:italic>\n                    \u2009=\u20097) corneas. Both methods showed reduced mechanical metrics in\n                    <jats:italic>Col14a1<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    corneas, with lower Brillouin frequency shift and lower OCE\u2010derived wave speed relative to WT controls. These findings support collagen XIV as a regulator of corneal stromal biomechanics and demonstrate the value of combining Brillouin microscopy with ACUS\u2010OCE for detecting biomechanical alterations in murine corneas.\n                  <\/jats:p>","DOI":"10.1002\/jbio.70329","type":"journal-article","created":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T10:48:42Z","timestamp":1785062922000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dual Optical Elastography Techniques Reveal the Regulation of Corneal Stiffness by Collagen\n                    <scp>XIV<\/scp>"],"prefix":"10.1002","volume":"19","author":[{"given":"Sajedeh Saeidi","family":"Fard","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering University of Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandeep","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8396-5451","authenticated-orcid":false,"given":"Manmohan","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander W.","family":"Schill","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Houston  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9530-1225","authenticated-orcid":false,"given":"Salavat R.","family":"Aglyamov","sequence":"additional","affiliation":[{"name":"Department of Biomedical 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