{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:09:42Z","timestamp":1789027782609,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Polarization\u2010resolved imaging provides a powerful, label\u2010free means of visualizing anisotropic structural order in biological tissue, yet its quantitative exploitation remains limited in routine microscopy. Here, we present a polarization\u2010resolved imaging approach based on polarized light microscopy (PLM) that enables automated, quantitative extraction of sarcomere\u2010scale structural metrics from unstained cardiac tissue sections. Using experimental rat models of acute cardiorespiratory arrest as controlled test systems, we demonstrate that polarization\u2010derived intensity profiles encode reproducible information on sarcomere length and band composition, with distinct patterns of structural compression observed across experimental conditions. Image\u2010derived measurements were validated against conventional histological and immunohistochemical staining, highlighting the complementary value of polarization contrast for rapid, staining\u2010free assessment of tissue anisotropy. Rather than addressing diagnostic specificity, this proof\u2010of\u2010concept study establishes PLM as a scalable quantitative imaging modality for polarization\u2010resolved analysis of hierarchical structural order in biological tissue, with potential integration into multimodal imaging workflows in biomedical research.<\/jats:p>","DOI":"10.1002\/jbio.70338","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T22:50:41Z","timestamp":1786402241000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Polarized Light Microscopy for Quantitative Assessment of Sarcomere\u2010Scale Structural Order in Cardiac Tissue"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7782-3468","authenticated-orcid":false,"given":"Gennadii","family":"Piavchenko","sequence":"first","affiliation":[{"name":"Human Anatomy and Histology Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artem","family":"Venediktov","sequence":"additional","affiliation":[{"name":"Human Anatomy and Histology Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoya","family":"Shamitko","sequence":"additional","affiliation":[{"name":"Human Anatomy and Histology Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3344-7890","authenticated-orcid":false,"given":"Karina","family":"Urazova","sequence":"additional","affiliation":[{"name":"Human Anatomy and Histology Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariia","family":"Obelchakova","sequence":"additional","affiliation":[{"name":"Human Anatomy and Histology Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9401-4986","authenticated-orcid":false,"given":"Evelina","family":"Khabarova","sequence":"additional","affiliation":[{"name":"Human Anatomy and Histology Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Ershov","sequence":"additional","affiliation":[{"name":"Pathophysiology Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Kartashkina","sequence":"additional","affiliation":[{"name":"Human Anatomy and Histology Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktoria","family":"Grikh","sequence":"additional","affiliation":[{"name":"Analytical, Physical and Colloid Chemistry Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerii","family":"Smirnov","sequence":"additional","affiliation":[{"name":"Pharmaceutical and Toxicological Chemistry Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina","family":"Ramenskaya","sequence":"additional","affiliation":[{"name":"Pharmaceutical and Toxicological Chemistry Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Yatskovskiy","sequence":"additional","affiliation":[{"name":"Human Anatomy and Histology Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7613-8191","authenticated-orcid":false,"given":"Igor","family":"Meglinski","sequence":"additional","affiliation":[{"name":"Human Anatomy and Histology Department I. M. Sechenov First Moscow State Medical University (Sechenov University)  Moscow Russia"},{"name":"College of Engineering and Physical Sciences Aston University  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-98600-w"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-16-1450-7_3"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41377-021-00639-x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsphotonics.0c01051"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786443408648474"},{"key":"e_1_2_10_7_1","first-page":"255","volume-title":"Advances in Oral Biology","author":"Carlstroem D.","year":"1964"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-45321-5"},{"key":"e_1_2_10_9_1","volume-title":"Current Protocols in Cell Biology","author":"Inou\u00e9 S.","year":"2002"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1051\/jeos\/2022011"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.28.10.102903"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.3469844"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2024288118"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.47093\/2218-7332.2022.13.4.18-32"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.2040-4603.2015.tb00664.x"},{"issue":"8","key":"e_1_2_10_16_1","first-page":"4467","article-title":"Elabela Alleviates Myocardial Ischemia Reperfusion\u2010Induced Apoptosis, Fibrosis and Mitochondrial Dysfunction Through PI3K\/AKT Signaling","volume":"12","author":"Yu P.","year":"2020","journal-title":"American Journal of Translational Research"},{"issue":"5","key":"e_1_2_10_17_1","first-page":"4254","article-title":"Hyperbaric Oxygen Protects Against Myocardial Ischemia\u2010Reperfusion Injury Through Inhibiting Mitochondria Dysfunction and Autophagy","volume":"22","author":"Chen W.","year":"2020","journal-title":"Molecular Medicine Reports"},{"issue":"2","key":"e_1_2_10_18_1","first-page":"2866","article-title":"Diagnosis of Sudden Cardiac Death due to Early Myocardial Ischemia: An Ultrastructural and Immunohistochemical Study","volume":"62","author":"Vison\u00e0 S. D.","year":"2018","journal-title":"European Journal of Histochemistry"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2016.03.009"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8100454"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.32794\/mr11250007"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00022.2018"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-15-4272-5_53"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.898623"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9929687"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2019.5097"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1013147524226"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(02)04686-6"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022969313879"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02445691"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-010-0520-y"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.83.1.237"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-6196(25)07683-9"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00094.2016"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00032.2012"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.314505"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06690-5"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00430621"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1999.0948"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00520.2005"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21082819"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.16552"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-022-01041-6"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-28985-3"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.105.071555"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-4328(00)00066-4"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.202213289"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1364\/OL.29.002031"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1088\/1612-202X\/aad857"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-017-2221-0"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2023.1118585"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2020.e03768"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAF.0b013e318177eab7"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2022.151993"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physchem-042018-052527"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2006.12.058"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1213\/00000539-199210000-00026"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-007-0584-4"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.70338","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbio.70338","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.70338","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T07:58:23Z","timestamp":1789027103000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.70338"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":57,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jbio.70338"],"URL":"https:\/\/doi.org\/10.1002\/jbio.70338","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-01-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70338"}}