{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:09:38Z","timestamp":1789027778404,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biophotonics"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hyperspectral imaging combined with plasmonic nanoparticles offers powerful opportunities for nanoscale biological diagnostics, yet conventional push\u2010broom systems are limited by slow acquisition and reduced spatial resolution. We report a wide\u2010field hyperspectral imaging platform integrating a custom linear variable tunable filter (LVTF) with reflected light microscopy (RLM) for multiplexed plasmonic nanoparticle (NP) imaging. While LVTF\u2010based systems have been used in industrial contexts, their adaptation to RLM microscopy enables high\u2010resolution, high\u2010contrast spectral imaging of NPs on biological samples. The system acquires data across 380\u2013720\u2009nm with 7\u201320\u2009nm spectral resolution while preserving spatial detail. We demonstrate imaging of NPs bound to cancer cell membranes and classification of four distinct NP types. A trained support vector machine achieves an overall\n                    <jats:italic>F<\/jats:italic>\n                    \u2010score of ~97%, significantly outperforming RGB\u2010based approaches. Compared to push\u2010broom systems, this platform provides faster acquisition, improved spatial resolution, and a compact, cost\u2010effective solution for multiplex NP detection in bioimaging and immunoplasmonic applications.\n                  <\/jats:p>","DOI":"10.1002\/jbio.70341","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T22:41:51Z","timestamp":1786488111000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Linear Variable Filter\u2010Based Wide\u2010Field Hyperspectral Camera for Multiplex Immunoplasmonics Imaging"],"prefix":"10.1002","volume":"19","author":[{"given":"C\u00e9cile","family":"Darviot","sequence":"first","affiliation":[{"name":"Department of Engineering Physics Polytechnique Montr\u00e9al  Montr\u00e9al Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2398-5602","authenticated-orcid":false,"given":"Michel","family":"Meunier","sequence":"additional","affiliation":[{"name":"Department of Engineering Physics Polytechnique Montr\u00e9al  Montr\u00e9al Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4NA00052H"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/c6nr01257d"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsanm.0c00376"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201900166"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202005400"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1115\/1.4038638"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","unstructured":"Y.Xu J.Chen L.Liyang andK. F. F.Kelly \u201cCompressive Hyperspectral Microscopy of Nanomaterials \u201d(2018) RAPID 2018\u20132018 IEEE Research and Applications of Photonics In Defense Conference no. Figure\u00a01 pp. 367\u2013369 https:\/\/doi.org\/10.1109\/RAPID.2018.8509015.","DOI":"10.1109\/RAPID.2018.8509015"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010008\u20102185\u20107"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010019\u201001620\u20109"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.OE.52.9.090901"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20311"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201400051"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1364\/OE.23.005102"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.OE.55.11.114105"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1364\/oe.20.000489"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/s21072276"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201400025"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.jbo.20.9.097001"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0034\u20104257(93)90024\u2010R"}],"container-title":["Journal of Biophotonics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.70341","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jbio.70341","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jbio.70341","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T07:58:34Z","timestamp":1789027114000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jbio.70341"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":19,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jbio.70341"],"URL":"https:\/\/doi.org\/10.1002\/jbio.70341","archive":["Portico"],"relation":{},"ISSN":["1864-063X","1864-0648"],"issn-type":[{"value":"1864-063X","type":"print"},{"value":"1864-0648","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70341"}}